EXTL3 Antibody (G-5): sc-271986

EXTL3 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects EXTL3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EXTL3 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EXTL3, also known as Reg receptor, EXT-related protein 1 (EXTR1), or glucuronyl-galactosyl-proteoglycan 4-α-N-acetylglucosaminyltransferase, is a crucial member of the EXT gene family, which is associated with tumor suppression and is involved in the biosynthesis of heparan sulfate (HS). EXTL3 is primarily localized to the endoplasmic reticulum membrane, where EXTL3 plays a vital role in the initiation and elongation of HS chains by adding N-acetylglucosamine (GlcNAc) to the polysaccharide-protein linkage region. EXTL3′s importance lies in developmental regulation and potential interactions with other proteins, such as binding to Reg via EXTL3′s N-terminus, which may influence various cellular processes and signaling pathways. The conservation of EXTL3′s C-terminus across species from C. elegans to vertebrates underscores the evolutionary significance in maintaining essential biological functions.

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EXTL3 Antibody (G-5) References:

1. Identification of a receptor for reg (regenerating gene) protein, a pancreatic beta-cell regeneration factor.  |  Kobayashi, S., et al. 2000. J Biol Chem. 275: 10723-6. PMID: 10753861
2. Overexpression of EXTL3/EXTR1 enhances NF-kappaB activity induced by TNF-alpha.  |  Mizuno, K., et al. 2001. Cell Signal. 13: 125-30. PMID: 11257457
3. Human tumor suppressor EXT gene family members EXTL1 and EXTL3 encode alpha 1,4- N-acetylglucosaminyltransferases that likely are involved in heparan sulfate/ heparin biosynthesis.  |  Kim, BT., et al. 2001. Proc Natl Acad Sci U S A. 98: 7176-81. PMID: 11390981
4. Alpha1,4-N-acetylglucosaminyltransferase encoding gene EXTL3 expression pattern in mouse adult and developing tissues with special attention to the pancreas.  |  Osman, NM., et al. 2003. Anat Embryol (Berl). 207: 333-41. PMID: 14618397
5. Glycosyltransferase encoding gene EXTL3 is differentially expressed in the developing and adult mouse cerebral cortex.  |  Osman, NM., et al. 2004. Brain Res Dev Brain Res. 151: 111-7. PMID: 15246697
6. Axon sorting in the optic tract requires HSPG synthesis by ext2 (dackel) and extl3 (boxer).  |  Lee, JS., et al. 2004. Neuron. 44: 947-60. PMID: 15603738
7. Heparan sulfate polymerization in Drosophila.  |  Izumikawa, T., et al. 2006. J Biol Chem. 281: 1929-34. PMID: 16303756
8. Expression of rib-1, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes, is indispensable for heparan sulfate synthesis and embryonic morphogenesis.  |  Kitagawa, H., et al. 2007. J Biol Chem. 282: 8533-44. PMID: 17237233
9. Contribution of EXT1, EXT2, and EXTL3 to heparan sulfate chain elongation.  |  Busse, M., et al. 2007. J Biol Chem. 282: 32802-10. PMID: 17761672
10. Identification of a third EXT-like gene (EXTL3) belonging to the EXT gene family.  |  Van Hul, W., et al. 1998. Genomics. 47: 230-7. PMID: 9479495

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EXTL3 Antibody (G-5) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-EXTL3 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EXTL3 Antibody (G-5) Specifications

Alternate Product Name: EXTL3

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: EXTL3

Epitope Species: human

Product Reactivity/Detection: EXTL3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for EXTL3 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin