EXT1 Antibody (A-7): sc-515144

EXT1 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects EXT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EXT1 (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EXT1 protein plays a crucial role in the biosynthesis of heparan sulfate, a glycosaminoglycan essential for various biological processes, including cell signaling, growth factor activity, and tissue development. By forming complexes with EXT2, EXT1 is localized in the Golgi apparatus, where EXT1 contributes to the polymerization of heparan sulfate, thereby influencing cellular interactions and the extracellular matrix. Mutations in EXT1 gene are associated with hereditary multiple exostoses, an autosomal dominant disorder characterized by the formation of cartilage-capped bony protrusions, which can lead to significant morbidity. EXT1′s ability to rescue defective heparan sulfate biosynthesis in mutated cells highlights EXT1′s importance in maintaining normal cellular functions and underscores anti-EXT1 antibody (A-7)′s relevance in research and diagnostic applications related to skeletal disorders and glycosaminoglycan metabolism.

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EXT1 Antibody (A-7) References:

1. The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate.  |  McCormick, C., et al. 2000. Proc Natl Acad Sci U S A. 97: 668-73. PMID: 10639137
2. Association of EXT1 and EXT2, hereditary multiple exostoses gene products, in Golgi apparatus.  |  Kobayashi, S., et al. 2000. Biochem Biophys Res Commun. 268: 860-7. PMID: 10679296
3. Molecular basis of multiple exostoses: mutations in the EXT1 and EXT2 genes.  |  Wuyts, W. and Van Hul, W. 2000. Hum Mutat. 15: 220-7. PMID: 10679937
4. Increased EXT1 gene copy number correlates with increased mRNA level predicts short disease-free survival in hepatocellular carcinoma without vascular invasion.  |  Dong, S., et al. 2018. Medicine (Baltimore). 97: e12625. PMID: 30278583
5. EXT1, Regulated by MiR-665, Promotes Cell Apoptosis via ERK1/2 Signaling Pathway in Acute Lymphoblastic Leukemia.  |  Liu, NW., et al. 2019. Med Sci Monit. 25: 6491-6503. PMID: 31465316
6. EXT1 methylation promotes proliferation and migration and predicts the clinical outcome of non-small cell lung carcinoma via WNT signalling pathway.  |  Kong, W., et al. 2021. J Cell Mol Med. 25: 2609-2620. PMID: 33565239
7. Obesity induced Ext1 reduction mediates the occurrence of NAFLD.  |  Wang, M., et al. 2022. Biochem Biophys Res Commun. 589: 123-130. PMID: 34906902
8. EXT1 and NCAM1-associated membranous lupus nephritis in a cohort of patients undergoing repeat kidney biopsies.  |  Miller, PP., et al. 2023. Nephrol Dial Transplant. 38: 396-404. PMID: 35278072
9. Exostoisns (EXT1/2) in Head and Neck Cancers: An In Silico Analysis and Clinical Correlates.  |  Wang, Y., et al. 2024. Int Dent J. 74: 446-453. PMID: 37989698
10. Generation of Ext1 Gene-Edited Mice Model Via Dual sgRNAs/Cas9 System and Phenotypic Analyses.  |  Zhou, L., et al. 2024. Mol Biotechnol.. PMID: 39565542
11. The putative tumour suppressor EXT1 alters the expression of cell-surface heparan sulfate.  |  McCormick, C., et al. 1998. Nat Genet. 19: 158-61. PMID: 9620772
12. The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate.  |  Lind, T., et al. 1998. J Biol Chem. 273: 26265-8. PMID: 9756849

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EXT1 Antibody (A-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-EXT1 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EXT1 Antibody (A-7) Specifications

Alternate Product Name: EXT1

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 219-332

Epitope Target: EXT1

Epitope Species: human

Product Reactivity/Detection: EXT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for EXT1 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin