Exportin 6 CRISPR/Cas9 KO Plasmid (h): sc-404915
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Exportin 6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Exportin 6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Exportin 6 CRISPR/Cas9 KO Plasmid (h) | sc-404915 | 20 µg | $397.00 |
Overview
XPO6 encodes exportin 6, a RanGTP-dependent nuclear export receptor that mediates the selective transport of profilin–actin complexes from the nucleus to the cytoplasm. By controlling nuclear actin availability, Exportin 6 influences actin-dependent chromatin remodeling, transcriptional regulation, and cell-cycle–linked nuclear architecture. This nucleocytoplasmic trafficking pathway interfaces with broader Ran/importin–exportin transport networks that coordinate gene expression programs and stress responses. Altered actin dynamics and nuclear transport regulation involving XPO6 have been studied in contexts relevant to tumor cell behavior and other disorders characterized by cytoskeletal and transcriptional dysregulation.
Exportin 6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the XPO6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the XPO6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the XPO6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Exportin 6 protein expression.
This CRISPR knockout system enables efficient generation of XPO6-deficient cell models for investigation of Exportin 6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting XPO6 exon(s) critical for Exportin 6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple XPO6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Exportin 6 CRISPR/Cas9 KO Plasmid (h) and Exportin 6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the XPO6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Exportin 6 HDR Plasmid (h) and Exportin 6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by XPO6 homology arms to support homology-directed repair at defined XPO6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.