Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Exportin 5 Double Nickase Plasmid (h): sc-403402-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Exportin 5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Exportin 5 Double Nickase Plasmid (h) and Exportin 5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XPO5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Exportin 5 Antibody (A-11): sc-271036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Exportin 5 Double Nickase Plasmid (h) | sc-403402-NIC | 20 µg | $410.00 | |||
Exportin 5 Double Nickase Plasmid (h2) | sc-403402-NIC-2 | 20 µg | $410.00 |
XPO5 encodes Exportin 5, a RanGTP-dependent nuclear export receptor that transports pre-miRNAs and other structured RNAs from the nucleus to the cytoplasm, enabling Dicer processing and mature microRNA biogenesis. By controlling the availability of miRNA precursors, Exportin 5 influences post-transcriptional gene regulation programs linked to cell cycle control, differentiation, and stress responses. XPO5 function intersects with RNA interference pathways and broader nucleocytoplasmic transport processes coordinated by the Ran system. Dysregulation of XPO5-dependent export has been associated with altered miRNA profiles observed in multiple disease-relevant contexts, supporting its study in mechanisms of gene expression control.
Exportin 5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XPO5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XPO5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XPO5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XPO5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.