Exo70 Double Nickase Plasmid (m): sc-424708-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Exo70 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Exo70 Double Nickase Plasmid (m) and Exo70 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Exoc7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Exo70 Antibody (D-6): sc-365825
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Exo70 Double Nickase Plasmid (m) | sc-424708-NIC | 20 µg | $410.00 | |||
Exo70 Double Nickase Plasmid (m2) | sc-424708-NIC-2 | 20 µg | $410.00 |
Exoc7 encodes Exo70, a core subunit of the octameric exocyst complex that tethers secretory vesicles to specific plasma membrane sites to enable polarized exocytosis. In mouse cells, Exo70 supports membrane trafficking events required for epithelial polarity, neurite outgrowth, cytokinesis, and directed cell migration by coordinating vesicle docking with actin cytoskeleton remodeling and small GTPase signaling pathways. Exocyst-dependent trafficking influences receptor recycling and surface delivery of adhesion molecules, linking Exo70 function to pathways that regulate cell shape, invasion, and tissue morphogenesis. Dysregulation of exocyst components has been associated with aberrant cell motility and developmental defects, making Exoc7 a useful target for mechanistic studies of trafficking-driven phenotypes.
Exo70 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Exoc7 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Exoc7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Exoc7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Exoc7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.