EWS Antibody (G-5): sc-28327
- EWS Antibody (G-5) is a mouse monoclonal IgG1 κ EWS antibody, cited in 38 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-19 at the N-terminus of EWS of human origin
- EWS Antibody (G-5) is recommended for detection of EWS of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- Anti-EWS Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EWS Antibody (G-5) for WB and IHC(P) applications. These reagents are now offered in bundles with EWS Antibody (G-5) (see ordering information below).
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SEE ALSO...
EWS Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects EWS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-EWS antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EWS protein is a nuclear RNA-binding protein that plays a crucial role in regulating gene expression and RNA processing. EWS protein′s importance is underscored by involvement in chromosomal translocations that lead to fusion proteins, such as EWS/Fli-1, which are implicated in the pathogenesis of Ewing sarcoma and other neoplasias. EWS/Fli-1 fusion protein acts as a potent transcriptional activator, enhancing the expression of target genes that promote cell proliferation and transformation. Understanding the functional dynamics of EWS and interactions with various transcription factors is vital for elucidating the molecular mechanisms underlying tumorigenesis, making anti-EWS antibody (G-5) an essential tool for researchers studying cancer biology and therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EWS Antibody (G-5) References:
- Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. | Delattre, O., et al. 1992. Nature. 359: 162-5. PMID: 1522903
- Molecular recognition by the EWS transcriptional activation domain. | Lee, KA. 2012. Adv Exp Med Biol. 725: 106-25. PMID: 22399321
- Targeting the EWS-ETS transcriptional program by BET bromodomain inhibition in Ewing sarcoma. | Hensel, T., et al. 2016. Oncotarget. 7: 1451-63. PMID: 26623725
- EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. | Gorthi, A., et al. 2018. Nature. 555: 387-391. PMID: 29513652
- TRIM8 modulates the EWS/FLI oncoprotein to promote survival in Ewing sarcoma. | Seong, BKA., et al. 2021. Cancer Cell. 39: 1262-1278.e7. PMID: 34329586
- A variant Ewing's sarcoma translocation (7;22) fuses the EWS gene to the ETS gene ETV1. | Jeon, IS., et al. 1995. Oncogene. 10: 1229-34. PMID: 7700648
- Multiple domains mediate transformation by the Ewing's sarcoma EWS/FLI-1 fusion gene. | Lessnick, SL., et al. 1995. Oncogene. 10: 423-31. PMID: 7845667
- A second Ewing's sarcoma translocation, t(21;22), fuses the EWS gene to another ETS-family transcription factor, ERG. | Sorensen, PH., et al. 1994. Nat Genet. 6: 146-51. PMID: 8162068
- The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1. | May, WA., et al. 1993. Mol Cell Biol. 13: 7393-8. PMID: 8246959
- The EWS-ATF-1 gene involved in malignant melanoma of soft parts with t(12;22) chromosome translocation, encodes a constitutive transcriptional activator. | Fujimura, Y., et al. 1996. Oncogene. 12: 159-67. PMID: 8552387
- EWS-ERG fusion transcript produced by chromosomal insertion in a Ewing sarcoma. | Kaneko, Y., et al. 1997. Genes Chromosomes Cancer. 18: 228-31. PMID: 9071576
- A new member of the ETS family fused to EWS in Ewing tumors. | Peter, M., et al. 1997. Oncogene. 14: 1159-64. PMID: 9121764
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EWS Antibody (G-5) | sc-28327 | 200 µg/ml | $322.00 | |||
EWS Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-528424 | 200 µg Ab; 10 µg BP | $361.00 | |||
EWS Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-520798 | 200 µg Ab, 40 µg BP | $361.00 | |||
EWS Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-542925 | 200 µg Ab; 20 µg BP | $361.00 | |||
EWS Antibody (G-5) AC | sc-28327 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EWS Antibody (G-5) HRP | sc-28327 HRP | 200 µg/ml | $322.00 | |||
EWS Antibody (G-5) FITC | sc-28327 FITC | 200 µg/ml | $336.00 | |||
EWS Antibody (G-5) PE | sc-28327 PE | 200 µg/ml | $349.00 | |||
EWS Antibody (G-5) Alexa Fluor® 488 | sc-28327 AF488 | 200 µg/ml | $364.00 | |||
EWS Antibody (G-5) Alexa Fluor® 546 | sc-28327 AF546 | 200 µg/ml | $364.00 | |||
EWS Antibody (G-5) Alexa Fluor® 594 | sc-28327 AF594 | 200 µg/ml | $364.00 | |||
EWS Antibody (G-5) Alexa Fluor® 647 | sc-28327 AF647 | 200 µg/ml | $364.00 | |||
EWS Antibody (G-5) Alexa Fluor® 680 | sc-28327 AF680 | 200 µg/ml | $364.00 | |||
EWS Antibody (G-5) Alexa Fluor® 790 | sc-28327 AF790 | 200 µg/ml | $364.00 | |||
EWS (G-5) Neutralizing Peptide | sc-28327 P | 100 µg/0.5 ml | $69.00 |