Ethenoadenosine Antibody (1G4): sc-52666

Ethenoadenosine Antibody (1G4) is a mouse monoclonal IgG2a antibody that detects Ethenoadenosine protein of NA origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Ethenoadenosine antibody (1G4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ethenoadenosine, a product of exposure to the carcinogen vinyl chloride, plays a crucial role in cellular signaling and metabolism, particularly through interaction with various nucleotide derivatives. Ethenoadenosine′s structure, characterized by the presence of an epsilon-adenine ring and variable phosphate groups, is essential for function as a signaling molecule, influencing processes such as energy transfer and cellular communication. Ethenoadenosine oligophosphates, including epsilon-ATP, epsilon-ADP, and epsilon-AMP, are utilized in research to explore complex biochemical pathways and mechanisms that remain poorly understood. Notably, epsilon-ADP is frequently employed to study skeletal muscle myosin, as epsilon-ADP binds to two independent sites on myosin, providing insights into muscle contraction and energy utilization.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ethenoadenosine Antibody (1G4) References:

1. A possible solvent effect of adenosine diphosphate influences the binding of 1,N6 ethenoadenosine diphosphate to myosin from skeletal muscle.  |  Grazi, E., et al. 2001. Biochim Biophys Acta. 1525: 130-5. PMID: 11342262
2. Receptor binding properties of di (1,N6-ethenoadenosine) 5', 5'''-P1, P4-tetraphosphate and its modulatory effect on extracellular glutamate levels in rat striatum.  |  Oaknin, S., et al. 2001. Neurosci Lett. 309: 177-80. PMID: 11514070
3. Flow cytometric and immunoblot assays for cell surface ADP-ribosylation using a monoclonal antibody specific for ethenoadenosine.  |  Krebs, C., et al. 2003. Anal Biochem. 314: 108-15. PMID: 12633608
4. Synthesis of an oligonucleotide analogue of ethenoadenosine.  |  Koissi, N. and Lönnberg, H. 2003. Nucleosides Nucleotides Nucleic Acids. 22: 1135-7. PMID: 14565363
5. [Covalent binding of 1,N(6)-ethenoadenosine diphosphate to catalytic and noncatalytic sites of chloroplast ATP-synthase].  |  Tatarintsev, NP. and Mal'ian, AN. 2006. Biofizika. 51: 282-7. PMID: 16637334
6. Fluorescence studies of the interaction between 1,N6-ethenoadenosine monophosphate and nucleotides.  |  Kubota, Y., et al. 1979. Biophys Chem. 9: 105-9. PMID: 218645
7. Development of techniques to monitor for exposure to vinyl chloride: monoclonal antibodies to ethenoadenosine and ethenocytidine.  |  Young, TL. and Santella, RM. 1988. Carcinogenesis. 9: 589-92. PMID: 3356066
8. Incorporation of 1,N6-ethenoadenosine into the 3' terminus of tRNA using T4 RNA ligase. 2. Preparation and ribosome interaction of fluorescent Escherichia coli tRNAMetf.  |  Paulsen, H. and Wintermeyer, W. 1984. Eur J Biochem. 138: 125-30. PMID: 6363067
9. Charge effects on the dynamic quenching of fluorescence of 1,N6-ethenoadenosine oligophosphates by iodide, thallium (I) and acrylamide.  |  Ando, T. and Asai, H. 1980. J Biochem. 88: 255-64. PMID: 7410337

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ethenoadenosine Antibody (1G4) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ethenoadenosine Antibody (1G4) Specifications

Alternate Product Name: Ethenoadenosine

Product Clone Name: 1G4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a lambda light chain

Amino Acids:

Epitope Target: Ethenoadenosine

Epitope Species: n/a

Product Reactivity/Detection: Ethenoadenosine

Species Reactivity/Detection: NA

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ethenoadenosine Antibody (1G4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin