ETFDH Antibody (D-2): sc-515202

ETFDH Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ETFDH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ETFDH antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ETFDH protein, also known as electron transfer flavoprotein-ubiquinone oxidoreductase, MADD, or ETFQO, is a crucial 617 amino acid membrane-bound electron transfer protein that plays a vital role in mitochondrial metabolism. ETFDH is localized to the inner mitochondrial membrane, where ETFDH functions as a monomer and belongs to the ETF-QO/fixC family. ETFDH is responsible for accepting electrons from electron-transfer flavoprotein (ETF) in the mitochondrial matrix and subsequently reducing ubiquinone in the mitochondrial membrane, a process essential for efficient energy production and metabolic regulation. ETFDH gene is located on human chromosome 4q32.1 and encodes a protein that contains one molecule of flavin adenine dinucleotide (FAD) and a 4Fe-4S cluster, which are critical for electron transfer activity. Alternative splicing of ETFDH gene results in two isoforms, which may have distinct functional roles. Defects in ETFDH are linked to glutaric aciduria type 2C (GA2C) or multiple acyl-CoA dehydrogenation deficiency (MADD), an autosomal recessive disorder characterized by severe metabolic disturbances, including hypoketotic hypoglycemia and acidosis, underscoring the importance of ETFDH in maintaining metabolic homeostasis.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ETFDH Antibody (D-2) References:

1. Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple acyl-CoA dehydrogenation deficiency.  |  Olsen, RK., et al. 2003. Hum Mutat. 22: 12-23. PMID: 12815589
2. ETFDH mutations as a major cause of riboflavin-responsive multiple acyl-CoA dehydrogenation deficiency.  |  Olsen, RK., et al. 2007. Brain. 130: 2045-54. PMID: 17584774
3. Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO).  |  Fielding, AJ., et al. 2008. J Magn Reson. 190: 222-32. PMID: 18037314
4. ETFDH mutations, CoQ10 levels, and respiratory chain activities in patients with riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency.  |  Liang, WC., et al. 2009. Neuromuscul Disord. 19: 212-6. PMID: 19249206
5. Novel mutations in ETFDH gene in Chinese patients with riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency.  |  Law, LK., et al. 2009. Clin Chim Acta. 404: 95-9. PMID: 19265687
6. Multiple acyl-CoA dehydrogenation deficiency (glutaric aciduria type II), congenital polycystic kidneys, and symmetric warty dysplasia of the cerebral cortex in two brothers. I. Clinical, metabolical, and biochemical findings.  |  Lehnert, W., et al. 1982. Eur J Pediatr. 139: 56-9. PMID: 7173259
7. Multiple acyl-CoA dehydrogenation deficiency (glutaric aciduria type II), congenital polycystic kidneys, and symmetric warty dysplasia of the cerebral cortex in two newborn brothers. II. Morphology and pathogenesis.  |  Böhm, N., et al. 1982. Eur J Pediatr. 139: 60-5. PMID: 7173260
8. Assignment of Etfdh, Etfb, and Etfa to chromosomes 3, 7, and 13: the mouse homologs of genes responsible for glutaric acidemia type II in human.  |  White, RA., et al. 1996. Genomics. 33: 131-4. PMID: 8617498

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ETFDH Antibody (D-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ETFDH Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ETFDH Antibody (D-2) Specifications

Alternate Product Name: ETFDH

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 302-600

Epitope Target: ETFDH

Epitope Species: human

Product Reactivity/Detection: ETFDH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ETFDH Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin