ETBR Antibody (5H2): sc-293198

ETBR Antibody (5H2) is a mouse monoclonal IgG2a kappa light chain antibody that detects ETBR protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ETBR antibody (5H2) is available as a non-conjugated format. The endothelin receptor B (ETBR), also known as EDNRB, ETB, ETRB, HSCR, and HSCR2, is a crucial member of the guanine-binding, regulatory protein-coupled receptor family, with three known isoforms: isoform 1, isoform 2, and δ3. ETBR is primarily located in various tissues, including lung, kidney, placenta, skeletal muscle, and stem villi vessels, where ETBR plays a vital role in regulating sodium excretion and glomerular filtration rate (GFR). ETBR presence in these tissues is essential for normal physiological functions, as ETBR contributes to the development of neural crest-derived cell lineages, including epidermal melanocytes and enteric neurons. Notably, both ETAR and ETBR receptors are activated by endothelin-1 (ET1), leading to significant physiological responses such as inhibition of sodium-potassium transport in the lens and an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. ETBR deficiencies can lead to early onset kidney dysfunction, characterized by reduced sodium excretion, decreased GFR, and slightly elevated blood pressure, while ETBR gene mutations are associated with congenital aganglionic megacolon and pigment abnormalities, including multigenic disorder Hirschsprung′s disease Type 2.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ETBR Antibody (5H2) References:

1. Endothelin receptor B protects granulocyte macrophage colony-stimulating factor mRNA from degradation.  |  Jungck, D., et al. 2015. J Pharmacol Exp Ther. 353: 564-72. PMID: 25855530
2. Downregulation of Endothelin Receptor B Contributes to Defective B Cell Lymphopoiesis in Trisomy 21 Pluripotent Stem Cells.  |  MacLean, GA., et al. 2018. Sci Rep. 8: 8001. PMID: 29789608
3. Knockdown of endothelin receptor B inhibits the progression of triple-negative breast cancer.  |  Gu, X., et al. 2019. Ann N Y Acad Sci. 1448: 5-18. PMID: 30900271
4. Endothelin receptor B affects the perfusion of newborn intestine: possible mechanism of necrotizing enterocolitis development.  |  Miyake, H., et al. 2019. Pediatr Surg Int. 35: 1339-1343. PMID: 31555862
5. Endothelin receptor B controls the production of fibroblast growth factor 23.  |  Feger, M., et al. 2020. FASEB J. 34: 6262-6270. PMID: 32157737
6. Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis.  |  Xu, G., et al. 2022. Sci Rep. 12: 19772. PMID: 36396948
7. Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin.  |  da Silva, CN., et al. 2023. Clin Cosmet Investig Dermatol. 16: 2847-2853. PMID: 37850109
8. ET-1 receptor type B (ETBR) overexpression associated with ICAM-1 downregulation leads to inflammatory attenuation in experimental autoimmune myocarditis.  |  Yang, P., et al. 2023. PeerJ. 11: e16320. PMID: 37901475
9. Endothelin receptor B-deficient mice are protected from high-fat diet-induced metabolic syndrome.  |  Feger, M., et al. 2024. Mol Metab. 80: 101868. PMID: 38159882
10. Endothelin receptor B is required for the blood pressure-lowering effect of G protein-coupled estrogen receptor 1 in ovariectomized rats.  |  Almutlaq, RN., et al. 2024. Am J Physiol Renal Physiol. 327: F599-F609. PMID: 39143913

The preferred ETBR antibody is ETBR Antibody (C-5): sc-518149.