Endothelin-3/EDN3/ET-3 Antibody (5E8): sc-81944

ET-3 Antibody (5E8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the ET-3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ET-3 antibody (5E8) is available as the non-conjugated format. The endothelin (ET) family of proteins, which includes ET-3 (endothelin 3), plays a crucial role in various physiological processes, particularly in the cardiovascular and nervous systems. ET-3 is a 238 amino acid secreted protein that is vital for proper neuronal development and is expressed in placental stem villi vessels and trophoblasts. ET-3 acts as a ligand for the endothelin receptor type B (ETBR), facilitating the maturation of enteric neurons and melanocytes. Notably, ET-3 undergoes significant post-translational modifications, resulting in the production of both a short active isoform and a longer inactive form, which is essential for regulating biological activity. Disruptions in the gene encoding ET-3 can lead to serious developmental disorders, including Hirschsprung disease type 1, congenital central hypoventilation syndrome, and Waardenburg syndrome type IV. ET-3′s ability to interact with specific receptors and involvement in critical developmental pathways underscore its importance in both health and disease, making ET-3 monoclonal antibody (5E8) a valuable tool for researchers studying these processes.

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Endothelin-3/EDN3/ET-3 Antibody (5E8) References:

1. Endothelin 3 induces the reversion of melanocytes to glia through a neural crest-derived glial-melanocytic progenitor.  |  Dupin, E., et al. 2000. Proc Natl Acad Sci U S A. 97: 7882-7. PMID: 10884419
2. A heterozygous endothelin 3 mutation in Waardenburg-Hirschsprung disease: is there a dosage effect of EDN3/EDNRB gene mutations on neurocristopathy phenotypes?  |  Pingault, V., et al. 2001. J Med Genet. 38: 205-9. PMID: 11303518
3. Endothelin-3 induces both human and opossum gallbladder contraction mediated mainly by endothelin-B receptor subtype in vitro.  |  Al-Jiffry, BO., et al. 2002. J Gastroenterol Hepatol. 17: 324-31. PMID: 11982704
4. Clinical relationship between EDN-3 gene, EDNRB gene and Hirschsprung's disease.  |  Duan, XL., et al. 2003. World J Gastroenterol. 9: 2839-42. PMID: 14669347
5. Endothelin-3-converting enzyme activity of the KEL1 and KEL6 phenotypes of the Kell blood group system.  |  Sha, Q., et al. 2006. J Biol Chem. 281: 7180-2. PMID: 16423827
6. Endothelin (ET)-1 and ET-3 promote expression of c-fos and c-jun in human choriocarcinoma via ET(B) receptor-mediated G(i)- and G(q)-pathways and MAP kinase activation.  |  Rauh, A., et al. 2008. Br J Pharmacol. 154: 13-24. PMID: 18362896
7. Epigenetic inactivation of endothelin-2 and endothelin-3 in colon cancer.  |  Wang, R., et al. 2013. Int J Cancer. 132: 1004-12. PMID: 22865632
8. Common mechanism in endothelin-3 and PAF receptor function for anti-inflammatory responses.  |  Sato, A. and Ebina, K. 2013. Eur J Pharmacol. 718: 30-3. PMID: 24055926
9. Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.  |  Wang, CH., et al. 2024. Nat Commun. 15: 7215. PMID: 39174539
10. A homozygous mutation in the endothelin-3 gene associated with a combined Waardenburg type 2 and Hirschsprung phenotype (Shah-Waardenburg syndrome).  |  Hofstra, RM., et al. 1996. Nat Genet. 12: 445-7. PMID: 8630503
11. Endothelin-3 frameshift mutation in congenital central hypoventilation syndrome.  |  Bolk, S., et al. 1996. Nat Genet. 13: 395-6. PMID: 8696331
12. Endothelin-3 gene mutations in isolated and syndromic Hirschsprung disease.  |  Bidaud, C., et al. 1997. Eur J Hum Genet. 5: 247-51. PMID: 9359047

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Endothelin-3/EDN3/ET-3 Antibody (5E8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Endothelin-3/EDN3/ET-3 Antibody (5E8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Endothelin-3/EDN3/ET-3 Antibody (5E8) Specifications

Alternate Product Name: ET-3

Product Clone Name: 5E8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-239

Epitope Target: ET-3

Epitope Species: human

Product Reactivity/Detection: ET-3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Endothelin-3/EDN3/ET-3 Antibody (5E8): N/A