Estrogen Receptor alpha Antibody (H222) recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB

Estrogen Receptor alpha Antibody (H222): sc-53492

Estrogen Receptor alpha Antibody (H222) is rated 4.0 out of 5 by 4.
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Datasheets

    • Estrogen Receptor alpha Antibody (H222) is a rat monoclonal IgG1 provided at 200 µg/ml
    • raised against Estrogen Receptor alpha of human origin
    • Estrogen Receptor alpha Antibody (H222) is recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB
    • Anti-Estrogen Receptor alpha Antibody (H222) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Estrogen Receptor alpha (H222): sc-53492.
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I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-02-17
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Rated 2 out of 5 by from Not good for Western blotting, but did work for IP I received a sample of this antibody, which I tested in 2 applications in MCF7 cell lysate: Western blotting and immunoprecipitation. I compared 6 different ERalpha antibodies in my optimization experiments, and this antibody was the only one that did not display any ERalpha (loaded 15 ug lysate) during Western blot visualization. However, when I perform immunoprecipitation with this antibody I see that ERalpha was successfully pulled down.
Date published: 2018-03-08
Rated 5 out of 5 by from Great! This antibody is a great selection for detection of ERα of mouse, rat and human origin by WB
Date published: 2017-01-23
Rated 5 out of 5 by from works well in WB WB in MCF7, a very specific band was observed in ~65 kd
Date published: 2016-12-27
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