Estrogen Receptor alpha Antibody (10H12B10) recommended for detection of Estrogen Receptor alpha of human origin by WB and ELISA

Estrogen Receptor alpha Antibody (10H12B10): sc-130072

Estrogen Receptor alpha Antibody (10H12B10) is rated 2.0 out of 5 by 1.
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Datasheets

    • Estrogen Receptor alpha Antibody (10H12B10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 301-595 of Estrogen Receptor alpha of human origin
    • recommended for detection of Estrogen Receptor alpha of human origin by WB and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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ER alpha (10H12B10) antibodies is it available for use in immunohistochemistry or can it be produced

Asked by: Shanthala
Thank you for your question. We do not currently recommend this specific clone for immunohistochemistry (IF or IHC(P)). There are several different monoclonal antibodies against ERalpha that we have tested and do recommend for this application. Please contact our Technical Service department for a product recommendation.
Answered by: Technical Support
Date published: 2019-02-02

How much sample do you recommend loading on the gel for Western blot detection using ERα (10H12B10): sc-130072?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 2 out of 5 by from Able to detect ER alpha in recombinant samples Able to detect ER alpha in recombinant samples, some smearing. -SCBT QC
Date published: 2013-11-03
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