Espin Antibody (E-7): sc-393469

Espin Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Espin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Espin antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Espin (ESPN), also known as autosomal recessive deafness type 36 protein (DFNB36), is an 854 amino acid cytoplasmic protein characterized by nine ankyrin repeats and one WH2 domain, which is crucial for function. The WH2 domain of ESPN plays a significant role in binding actin monomers, facilitating the assembly of actin bundles essential for structural integrity and signaling capabilities of actin-rich cellular specializations. This function is particularly important in sensory cells, where actin cytoskeleton organization is vital for effective sensory transduction. Mutations or defects in ESPN can lead to non-syndromic sensorineural deafness autosomal recessive type 36 (DFNB36), a condition resulting from damage to inner ear neural receptors or associated nerve pathways. In humans, ESPN is expressed as two isoforms due to alternative splicing, while in rodents, four major isoforms ranging from approximately 110 to 25 kDa have been identified, along with potential additional splice variants. ESPN has been shown to interact with proteins such as IRSp53 and Profilin-2, highlighting a role in cellular signaling and cytoskeletal dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Espin Antibody (E-7) References:

1. The deaf jerker mouse has a mutation in the gene encoding the espin actin-bundling proteins of hair cell stereocilia and lacks espins.  |  Zheng, L., et al. 2000. Cell. 102: 377-85. PMID: 10975527
2. Mutations of ESPN cause autosomal recessive deafness and vestibular dysfunction.  |  Naz, S., et al. 2004. J Med Genet. 41: 591-5. PMID: 15286153
3. Espin gene (ESPN) mutations associated with autosomal dominant hearing loss cause defects in microvillar elongation or organisation.  |  Donaudy, F., et al. 2006. J Med Genet. 43: 157-61. PMID: 15930085
4. Balanced levels of Espin are critical for stereociliary growth and length maintenance.  |  Rzadzinska, A., et al. 2005. Cell Motil Cytoskeleton. 62: 157-65. PMID: 16206170
5. Differential expression of espin isoforms during epithelial morphogenesis, stereociliogenesis and postnatal maturation in the developing inner ear.  |  Sekerková, G., et al. 2006. Dev Biol. 291: 83-95. PMID: 16413524
6. Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells.  |  Loomis, PA., et al. 2006. J Cell Sci. 119: 1655-65. PMID: 16569662
7. Structural polymorphism of the actin-espin system: a prototypical system of filaments and linkers in stereocilia.  |  Purdy, KR., et al. 2007. Phys Rev Lett. 98: 058105. PMID: 17358907
8. Microvillar size and espin expression in principal cells of the adult rat epididymis are regulated by androgens.  |  Primiani, N., et al. 2007. J Androl. 28: 659-69. PMID: 17409466
9. A novel mutation in the Espin gene causes autosomal recessive nonsyndromic hearing loss but no apparent vestibular dysfunction in a Moroccan family.  |  Boulouiz, R., et al. 2008. Am J Med Genet A. 146A: 3086-9. PMID: 18973245
10. An actin-binding protein espin is a growth regulator for melanoma.  |  Yanagishita, T., et al. 2014. J Invest Dermatol. 134: 2996-2999. PMID: 24936833
11. Lhx1/5 control dendritogenesis and spine morphogenesis of Purkinje cells via regulation of Espin.  |  Lui, NC., et al. 2017. Nat Commun. 8: 15079. PMID: 28516904
12. Identification and characterization of espin, an actin-binding protein localized to the F-actin-rich junctional plaques of Sertoli cell ectoplasmic specializations.  |  Bartles, JR., et al. 1996. J Cell Sci. 109 (Pt 6): 1229-39. PMID: 8799813

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Espin Antibody (E-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Espin Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Espin Antibody (E-7) Specifications

Alternate Product Name: Espin

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 645-767

Epitope Target: Espin

Epitope Species: human

Product Reactivity/Detection: Espin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Espin Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin