ESD Antibody (A-5): sc-514892
- ESD Antibody (A-5) is a mouse monoclonal IgG2b κ ESD antibody provided at 200 µg/ml
- raised against amino acids 1-282 representing full length ESD of human origin
- ESD Antibody (A-5) is recommended for detection of ESD of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ESD Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ESD Antibody (A-5). This work is in progress.
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SEE ALSO...
ESD Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects ESD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ESD antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ESD, or esterase D, is a 282 amino acid protein that plays a crucial role in detoxifying formaldehyde, a toxic compound that can cause cellular damage. ESD is primarily localized in the cytoplasm and cytoplasmic vesicles, with high expression levels found in the placenta, kidney, liver, and erythrocytes. The detoxification process involves the oxidation of formaldehyde by alcohol dehydrogenase 5, which produces S-formylglutathione, subsequently hydrolyzed by ESD into reduced forms of formic acid and glutathione. This enzymatic activity is vital for cellular health, as ESD helps mitigate the harmful effects of formaldehyde while also providing energy. Additionally, ESD has the ability to hydrolyze various neutral ester substrates and may function as a cysteine hydrolase, although enzymatic activity can be inhibited by thiol alkylating agents. Genetic polymorphisms in the ESD gene can lead to decreased enzymatic function, potentially increasing susceptibility to conditions such as toxic liver cirrhosis, retinoblastoma, obesity, and autism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ESD Antibody (A-5) References:
- Physiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidinii. | Yurimoto, H., et al. 2003. Microbiology (Reading). 149: 1971-1979. PMID: 12904537
- Purification, crystallization and preliminary X-ray diffraction analysis of S-formylglutathione hydrolase from Arabidopsis thaliana: effects of pressure and selenomethionine substitution on space-group changes. | McAuley, KE., et al. 2003. Acta Crystallogr D Biol Crystallogr. 59: 2272-4. PMID: 14646092
- Molecular basis of ESD*5 and ESD*7 and haplotype analysis with new polymorphisms in introns. | Yuasa, I., et al. 2004. Hum Biol. 76: 479-88. PMID: 15481680
- Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG. | Gonzalez, CF., et al. 2006. J Biol Chem. 281: 14514-22. PMID: 16567800
- Unique regulation of the active site of the serine esterase S-formylglutathione hydrolase. | Cummins, I., et al. 2006. J Mol Biol. 359: 422-32. PMID: 16626737
- Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens. | Okunuki, Y., et al. 2008. Mol Vis. 14: 1094-104. PMID: 18552983
- Structural characterization and reversal of the natural organophosphate resistance of a D-type esterase, Saccharomyces cerevisiae S-formylglutathione hydrolase. | Legler, PM., et al. 2008. Biochemistry. 47: 9592-601. PMID: 18707125
- S-formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification? | Harms, N., et al. 1996. J Bacteriol. 178: 6296-9. PMID: 8892832
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ESD Antibody (A-5) | sc-514892 | 200 µg/ml | $322.00 | |||
ESD Antibody (A-5) AC | sc-514892 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ESD Antibody (A-5) HRP | sc-514892 HRP | 200 µg/ml | $322.00 | |||
ESD Antibody (A-5) FITC | sc-514892 FITC | 200 µg/ml | $336.00 | |||
ESD Antibody (A-5) PE | sc-514892 PE | 200 µg/ml | $349.00 | |||
ESD Antibody (A-5) Alexa Fluor® 488 | sc-514892 AF488 | 200 µg/ml | $364.00 | |||
ESD Antibody (A-5) Alexa Fluor® 546 | sc-514892 AF546 | 200 µg/ml | $364.00 | |||
ESD Antibody (A-5) Alexa Fluor® 594 | sc-514892 AF594 | 200 µg/ml | $364.00 | |||
ESD Antibody (A-5) Alexa Fluor® 647 | sc-514892 AF647 | 200 µg/ml | $364.00 | |||
ESD Antibody (A-5) Alexa Fluor® 680 | sc-514892 AF680 | 200 µg/ml | $364.00 | |||
ESD Antibody (A-5) Alexa Fluor® 790 | sc-514892 AF790 | 200 µg/ml | $364.00 |