Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERRγ CRISPR/Cas9 KO Plasmid (h): sc-416300
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERRγ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ERRγ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERRγ CRISPR/Cas9 KO Plasmid (h) | sc-416300 | 20 µg | $397.00 |
Overview
ESRRG encodes estrogen-related receptor gamma (ERRγ), an orphan nuclear receptor that functions as a ligand-independent transcription factor regulating mitochondrial biogenesis, oxidative phosphorylation, and cellular energy homeostasis. ERRγ cooperates with PGC-1 coactivators to control gene programs involved in fatty acid oxidation, TCA cycle activity, and respiratory chain assembly, linking transcriptional control to metabolic adaptation. It also participates in tissue-specific differentiation programs, including cardiac and neuronal contexts, and modulates endocrine and stress-responsive signaling networks. Dysregulated ESRRG/ERRγ activity has been associated with metabolic remodeling and altered bioenergetic states observed across multiple disease-relevant models, supporting its use as a mechanistic node in systems biology studies.
ERRγ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ESRRG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ESRRG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ESRRG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ERRγ protein expression.
This CRISPR knockout system enables efficient generation of ESRRG-deficient cell models for investigation of ERRγ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ESRRG exon(s) critical for ERRγ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ESRRG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ERRγ CRISPR/Cas9 KO Plasmid (h) and ERRγ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ESRRG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ERRγ HDR Plasmid (h) and ERRγ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ESRRG homology arms to support homology-directed repair at defined ESRRG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.