ERRβ CRISPR/Cas9 KO Plasmid (m): sc-424021
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERRβ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ERRβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERRβ CRISPR/Cas9 KO Plasmid (m) | sc-424021 | 20 µg | $397.00 |
Overview
Estrogen-related receptor beta (ERRβ), encoded by mouse Esrrb, is an orphan nuclear receptor that functions as a transcription factor coordinating gene programs involved in energy metabolism, mitochondrial function, and cell fate decisions. In pluripotent cells, Esrrb contributes to maintenance of self-renewal and interfaces with core transcriptional networks that regulate differentiation and lineage commitment. ERRβ activity is linked to steroid hormone receptor signaling and broader transcriptional control of oxidative and developmental pathways. Dysregulation of Esrrb-dependent transcription has been associated with altered developmental outcomes and context-dependent changes in cellular proliferation and metabolic state, supporting its use as a mechanistic node in disease-relevant models.
ERRβ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Esrrb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Esrrb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Esrrb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ERRβ protein expression.
This CRISPR knockout system enables efficient generation of Esrrb-deficient cell models for investigation of ERRβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Esrrb exon(s) critical for ERRβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Esrrb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ERRβ CRISPR/Cas9 KO Plasmid (m) and ERRβ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Esrrb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ERRβ HDR Plasmid (m) and ERRβ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Esrrb homology arms to support homology-directed repair at defined Esrrb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.