Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERp57 Double Nickase Plasmid (m): sc-420698-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERp57 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ERp57 Double Nickase Plasmid (m) and ERp57 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pdia3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ERp57 Antibody (MaP.ERp57): sc-23886
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERp57 Double Nickase Plasmid (m) | sc-420698-NIC | 20 µg | $410.00 | |||
ERp57 Double Nickase Plasmid (m2) | sc-420698-NIC-2 | 20 µg | $410.00 |
Mouse Pdia3 encodes ERp57 (PDIA3), an endoplasmic reticulum protein disulfide isomerase that cooperates with calnexin and calreticulin to catalyze oxidative folding and quality control of nascent glycoproteins. ERp57 activity supports ER proteostasis, disulfide bond formation, and signaling linked to ER stress and the unfolded protein response, influencing antigen presentation through MHC class I peptide loading. Dysregulated PDIA3/ERp57 function has been associated with altered redox balance, proteotoxic stress responses, and pathways implicated in neurodegeneration, metabolic dysfunction, and tumor biology. As a multifunctional oxidoreductase, ERp57 is also studied for roles in calcium homeostasis, cell adhesion-related signaling, and stress-adaptive transcriptional programs.
ERp57 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pdia3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pdia3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pdia3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pdia3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.