Anti-ERK 2 Antibody (D-2) recommended for detection of ERK 2 p42 of mouse, rat, human and origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-ERK 2 Antibody (D-2): sc-1647

Anti-ERK 2 Antibody (D-2) is rated 4.8 out of 5 by 27.
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Datasheets

    • ERK 2 Antibody (D-2) is a mouse monoclonal IgG2b κ, cited in 499 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 345-358 at the C-terminus of ERK 2 of human origin
    • ERK 2 Antibody (D-2) is recommended for detection of ERK 2 p42 of mouse, rat, human and origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
    • Anti-ERK 2 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • available conjugated to biotin for WB, IHC(P) and ELISA; and to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERK 2 (D-2): sc-1647.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for ERK 2 Antibody (D-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with ERK 2 Antibody (D-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Hi, does this ERK2 ab detect both unphosphorylated and MEK-phosphorylated ERK2?

Asked by: CharlesF
Thank you for your question. Yes, this is a total ERK 2 antibody and can detect both phosphorylated and un-phosphorylated ERK 2. Please refer to the Western blot data on our website for further evidence of this reactivity.
Answered by: Technical Support
Date published: 2019-05-20

What is the evidence that this mAb is actually specific to ERK2 and does *not* bind (or minimally binds) ERK1? The given immunizing sequence (ERK2 345-358) shares 12/13 amino acid residues with ERK1 so it is a little hard to believe this is ERK2-specific.

Asked by: Will Herrick
Thank you for your inquiry. Since we do not epitope map our monoclonal antibodies, we cannot say with certainty if this clone is cross-reactive with ERK 1, but there is a good possibility that it is reactive with ERK 1 p44.
Answered by: Technical Service
Date published: 2019-05-29
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Rated 5 out of 5 by from Works well in western blot This antibody works well in western blot of mouse liver samples
Date published: 2021-03-22
Rated 5 out of 5 by from Excellent A great antibody for WB analysis with specific binding and a clear signal.
Date published: 2021-03-22
Rated 5 out of 5 by from ERK 2 Antibody (D-2) This antibody has worked well in West blotting.Excellent results.
Date published: 2020-10-27
Rated 5 out of 5 by from Good for WB We used it at 1:500 dilution and it specifically detected endogenous human ERK2.
Date published: 2020-06-25
Rated 5 out of 5 by from sc-1647 Review Worked well as a primary antibody used for a western blot.
Date published: 2020-04-10
Rated 5 out of 5 by from Anti-ERK2 for Western Blot Analysis Anti-ERK2 (D2) preformed well as a primary antibody for western blot analysis and supernatant collection.
Date published: 2019-12-12
Rated 5 out of 5 by from Perfect I've tried a sample requested to substitute the now discontinued rabbit ERK2 antibody. It works very well. Used 1:1000 in tbs 0,1%tween . One band at 42KDa
Date published: 2019-09-17
Rated 5 out of 5 by from excellent for WB High sensitivity (better sensitivity than Erk1/2 C-9), and essentially no non-specific bands (very weak non-specific signal at ~300 kDa, negligible) for WB. Specific to Erk2, no reactivity with Erk1. Go to Erk1/2 C-9 if you need detect both.
Date published: 2019-05-29
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