ERdj3 Antibody (C-7): sc-271240
- ERdj3 Antibody (C-7) is a mouse monoclonal IgG1 κ ERdj3 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 106-235 mapping within an internal region of ERdj3 of human origin
- ERdj3 Antibody (C-7) is recommended for detection of mature ERdj3 and DnaJ B11 precursor of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-ERdj3 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERdj3 (C-7): sc-271240.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for ERdj3 Antibody (C-7) for WB applications. This reagent is now offered in a bundle with ERdj3 Antibody (C-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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ERdj3 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ERdj3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ERdj3 antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERdj3, a member of the heat shock protein 40 (HSP 40) family, plays a crucial role in the endoplasmic reticulum (ER) by assisting in the proper folding and maturation of nascent polypeptides. ERdj3′s location within the ER ensures misfolded proteins are recognized and targeted for degradation, maintaining cellular homeostasis and preventing toxic aggregate accumulation. ERdj3 interacts with BiP, a key chaperone involved in protein translocation and quality control, and associates with various substrates, including unfolded immunoglobulin light chains and viral proteins. ERdj3 is implicated in the retrotranslocation process of proteins, such as those affected by Shiga toxin, which hijacks cellular machinery to promote cytoplasmic entry. Through facilitating these critical processes, ERdj3 contributes significantly to the ER quality control system, highlighting ERdj3′s importance in cellular health and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ERdj3 Antibody (C-7) References:
- HEDJ, an Hsp40 co-chaperone localized to the endoplasmic reticulum of human cells. | Yu, M., et al. 2000. J Biol Chem. 275: 24984-92. PMID: 10827079
- A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins. | Meunier, L., et al. 2002. Mol Biol Cell. 13: 4456-69. PMID: 12475965
- ERdj3, a stress-inducible endoplasmic reticulum DnaJ homologue, serves as a cofactor for BiP's interactions with unfolded substrates. | Shen, Y. and Hendershot, LM. 2005. Mol Biol Cell. 16: 40-50. PMID: 15525676
- Localization and function in endoplasmic reticulum stress tolerance of ERdj3, a new member of Hsp40 family protein. | Nakanishi, K., et al. 2004. Cell Stress Chaperones. 9: 253-64. PMID: 15544163
- Shiga toxin is transported from the endoplasmic reticulum following interaction with the luminal chaperone HEDJ/ERdj3. | Yu, M. and Haslam, DB. 2005. Infect Immun. 73: 2524-32. PMID: 15784599
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERdj3 Antibody (C-7) | sc-271240 | 200 µg/ml | $316.00 | |||
ERdj3 Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-521888 | 200 µg Ab, 40 µg BP | $354.00 | |||
ERdj3 Antibody (C-7) AC | sc-271240 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ERdj3 Antibody (C-7) HRP | sc-271240 HRP | 200 µg/ml | $316.00 | |||
ERdj3 Antibody (C-7) FITC | sc-271240 FITC | 200 µg/ml | $330.00 | |||
ERdj3 Antibody (C-7) PE | sc-271240 PE | 200 µg/ml | $343.00 | |||
ERdj3 Antibody (C-7) Alexa Fluor® 488 | sc-271240 AF488 | 200 µg/ml | $357.00 | |||
ERdj3 Antibody (C-7) Alexa Fluor® 546 | sc-271240 AF546 | 200 µg/ml | $357.00 | |||
ERdj3 Antibody (C-7) Alexa Fluor® 594 | sc-271240 AF594 | 200 µg/ml | $357.00 | |||
ERdj3 Antibody (C-7) Alexa Fluor® 647 | sc-271240 AF647 | 200 µg/ml | $357.00 | |||
ERdj3 Antibody (C-7) Alexa Fluor® 680 | sc-271240 AF680 | 200 µg/ml | $357.00 | |||
ERdj3 Antibody (C-7) Alexa Fluor® 790 | sc-271240 AF790 | 200 µg/ml | $357.00 |