Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ErbB4/HER4 CRISPR/Cas9 KO Plasmid (h): sc-400275
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ErbB4/HER4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ErbB4/HER4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ErbB4/HER4 Antibody (C-7): sc-8050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ErbB4/HER4 CRISPR/Cas9 KO Plasmid (h) | sc-400275 | 20 µg | $397.00 |
Overview
ERBB4 encodes the receptor tyrosine kinase ErbB4/HER4, a member of the EGFR/ErbB family that mediates signaling downstream of neuregulins and other ligands. Upon ligand-induced dimerization and autophosphorylation, ErbB4 engages MAPK/ERK, PI3K–AKT, JAK/STAT, and PLCγ pathways to regulate cell fate decisions including proliferation, differentiation, migration, and survival. ERBB4 activity is also linked to receptor endocytosis and proteolytic processing that can modulate nuclear signaling outputs in specific cellular contexts. Dysregulated ERBB4 signaling and altered expression or isoform usage have been associated with oncogenic signaling networks and disease-relevant phenotypes in epithelial, neural, and cardiac biology.
ErbB4/HER4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ERBB4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ERBB4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ERBB4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ErbB4/HER4 protein expression.
This CRISPR knockout system enables efficient generation of ERBB4-deficient cell models for investigation of ErbB4/HER4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ERBB4 exon(s) critical for ErbB4/HER4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ERBB4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ErbB4/HER4 CRISPR/Cas9 KO Plasmid (h) and ErbB4/HER4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ERBB4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ErbB4/HER4 HDR Plasmid (h) and ErbB4/HER4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ERBB4 homology arms to support homology-directed repair at defined ERBB4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.