ErbB4/HER4 Antibody (D-10): sc-515142

ErbB-4 Antibody (D-10) is a mouse monoclonal IgG2b antibody that detects ErbB-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ErbB-4, a member of the epidermal growth factor receptor family, plays a crucial role in cell signaling pathways that regulate cell proliferation and differentiation, making ErbB-4 a significant target in cancer research, particularly in breast carcinoma. ErbB-4 is primarily located on the cell membrane, where ErbB-4 can form homodimers or heterodimers upon ligand binding, which is essential for activation and subsequent signaling cascades. Notably, the gene encoding ErbB-4 produces a full-length protein that is cleaved to generate a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment; the short fragment is heavily phosphorylated on tyrosine residues and exhibits catalytic activity, further emphasizing the importance of structural integrity in mediating cellular responses. ErbB-4 expression is particularly high in breast carcinoma cell lines, as well as in normal tissues such as skeletal muscle, heart, pituitary, brain, and cerebellum, highlighting ErbB-4′s relevance in both normal physiology and pathological conditions.

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ErbB4/HER4 Antibody (D-10) References:

1. Roles of ErbB-3 and ErbB-4 in the physiology and pathology of the mammary gland.  |  Carraway, KL., et al. 1997. J Mammary Gland Biol Neoplasia. 2: 187-98. PMID: 10882304
2. ErbB-4 and neuregulin expression in the adult mouse olfactory bulb after peripheral denervation.  |  Oberto, M., et al. 2001. Eur J Neurosci. 14: 513-21. PMID: 11553301
3. ErbB-4 expression in limb soft-tissue sarcoma: correlation with the results of neoadjuvant chemotherapy.  |  Merimsky, O., et al. 2002. Eur J Cancer. 38: 1335-42. PMID: 12091063
4. erbB-1 and erbB-4 receptors act in concert to facilitate female sexual development and mature reproductive function.  |  Prevot, V., et al. 2005. Endocrinology. 146: 1465-72. PMID: 15591145
5. ErbB-4 and TNF-alpha converting enzyme localization to membrane microdomains.  |  Thiel, KW. and Carpenter, G. 2006. Biochem Biophys Res Commun. 350: 629-33. PMID: 17027649
6. ErbB-4 may control behavior of prostate cancer cells and serve as a target for molecular therapy.  |  Ben-Yosef, R., et al. 2007. Prostate. 67: 871-80. PMID: 17440944
7. Targeting ErbB-1 and ErbB-4 in irradiated head and neck cancer: results of in vitro and in vivo studies.  |  Barnea, I., et al. 2013. Head Neck. 35: 399-407. PMID: 22367849
8. ErbB-3 and ErbB-4 function as the respective low and high affinity receptors of all Neu differentiation factor/heregulin isoforms.  |  Tzahar, E., et al. 1994. J Biol Chem. 269: 25226-33. PMID: 7929212
9. ErbB-3 and ErbB-4 expression in the mouse olfactory system.  |  Perroteau, I., et al. 1998. Ann N Y Acad Sci. 855: 255-9. PMID: 9929617
10. ErbB-4 mRNA expression is decreased in non-metastatic pancreatic cancer.  |  Graber, HU., et al. 1999. Int J Cancer. 84: 24-7. PMID: 9988227

The preferred ErbB4/HER4 antibody is ErbB4/HER4 Antibody (C-7): sc-8050.