epsin 1 Antibody (G-12): sc-48372

Epsin 1 Antibody (G-12) is a mouse monoclonal IgG2a antibody that detects Epsin 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Epsin 1 plays a crucial role in the endocytic process, particularly in the formation of Clathrin-coated vesicles, which are essential for the internalization of membrane proteins and lipids. Epsin 1 is primarily located at the leading edge of vesicular coated pits, where Epsin 1 facilitates the bending of the membrane, a critical step in endocytosis. The presence of an epsin N-terminal homology (ENTH) domain and a Clathrin-binding motif within Epsin 1 underscores Epsin 1′s importance in the recruitment of Clathrin and other accessory proteins necessary for vesicle formation. Additionally, Epsin 1 undergoes post-translational modifications, including phosphorylation, which can regulate Epsin 1′s activity and interactions with other proteins, thereby influencing the efficiency of endocytosis. Anti-Epsin 1 antibody (G-12) enables researchers to gain insights into the dynamics of endocytosis and the molecular mechanisms underlying synaptic transmission and cellular signaling.

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epsin 1 Antibody (G-12) References:

1. Epsin 1 undergoes nucleocytosolic shuttling and its eps15 interactor NH(2)-terminal homology (ENTH) domain, structurally similar to Armadillo and HEAT repeats, interacts with the transcription factor promyelocytic leukemia Zn(2)+ finger protein (PLZF).  |  Hyman, J., et al. 2000. J Cell Biol. 149: 537-46. PMID: 10791968
2. Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus.  |  Chen, C. and Zhuang, X. 2008. Proc Natl Acad Sci U S A. 105: 11790-5. PMID: 18689690
3. Epsin 1 is involved in recruitment of ubiquitinated EGF receptors into clathrin-coated pits.  |  Kazazic, M., et al. 2009. Traffic. 10: 235-45. PMID: 19054389
4. Embryonic arrest at midgestation and disruption of Notch signaling produced by the absence of both epsin 1 and epsin 2 in mice.  |  Chen, H., et al. 2009. Proc Natl Acad Sci U S A. 106: 13838-43. PMID: 19666558
5. Molecular basis of the potent membrane-remodeling activity of the epsin 1 N-terminal homology domain.  |  Yoon, Y., et al. 2010. J Biol Chem. 285: 531-40. PMID: 19880963
6. Adaptor protein complex-2 (AP-2) and epsin-1 mediate protease-activated receptor-1 internalization via phosphorylation- and ubiquitination-dependent sorting signals.  |  Chen, B., et al. 2011. J Biol Chem. 286: 40760-70. PMID: 21965661
7. Interaction with epsin 1 regulates the constitutive clathrin-dependent internalization of ErbB3.  |  Szymanska, M., et al. 2016. Biochim Biophys Acta. 1863: 1179-88. PMID: 26975582
8. The N-terminal homology (ENTH) domain of Epsin 1 is a sensitive reporter of physiological PI(4,5)P2 dynamics.  |  Leitner, MG., et al. 2019. Biochim Biophys Acta Mol Cell Biol Lipids. 1864: 433-442. PMID: 30670192
9. Cooperativity of membrane-protein and protein-protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis.  |  Kroppen, B., et al. 2021. Cell Mol Life Sci. 78: 2355-2370. PMID: 32997199
10. Epsin1-mediated exosomal sorting of Dll4 modulates the tubular-macrophage crosstalk in diabetic nephropathy.  |  Liu, JL., et al. 2023. Mol Ther. 31: 1451-1467. PMID: 37016580

The preferred epsin 1 antibody is epsin 1 Antibody (C-11): sc-55556.