Anti-ephrin-B1 Antibody (C-6) is a mouse monoclonal IgG1 (kappa light chain) ephrin-B1 antibody provided at 200 µg/ml
raised against amino acids 171-240 mapping near the C-terminus of ephrin-B1 of human origin
ephrin-B1 Antibody (C-6) is recommended for detection of ephrin-B1 of human origin by WB, IP, IF and ELISA
Anti-ephrin-B1 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ephrin-B1 (C-6): sc-515264.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ephrin-B1 Antibody (C-6) is a high quality monoclonal ephrin-B1 antibody (also designated ephrin-B1 antibody) suitable for the detection of the ephrin-B1 protein of human origin. ephrin-B1 Antibody (C-6) is available as both the non-conjugated anti-ephrin-B1 antibody form, as well as multiple conjugated forms of anti-ephrin-B1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B, based on their structure and function. Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Has ephrin-B1 Antibody (C-6): sc-515264 , been tested flowcytometry applications?
Asked by: hansas
Thank you for your question. We have not yet tested this mouse monoclonal antibody, ephrin-B1 (C-6): sc-515264, for flow cytometry, so this is not a recommended application at this time.
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to receive a free sample to test in this application. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-07-28
What is the recommended starting dilution for Western blot for sc-515264: ephrin-B1 (C-6) monoclonal antibody?
Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn