ephrin-B1 Antibody (C-6): sc-515264
- ephrin-B1 Antibody (C-6) is a mouse monoclonal IgG1 κ ephrin-B1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 171-240 mapping near the C-terminus of ephrin-B1 of human origin
- ephrin-B1 Antibody (C-6) is recommended for detection of ephrin-B1 of human origin by WB, IP, IF and ELISA
- Anti-ephrin-B1 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ephrin-B1 Antibody (C-6) for WB applications. These reagents are now offered in bundles with ephrin-B1 Antibody (C-6) (see ordering information below).
ephrin-B1 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ephrin-B1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ephrin-B1 monoclonal antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ephrins, which serve as ligands for Eph receptors, are critical cell-surface proteins categorized into ephrin-A and ephrin-B based on their structural and functional characteristics. ephrin-B1 is a transmembrane protein that features conserved cytoplasmic tyrosine residues, which undergo phosphorylation upon binding to EphB receptors, thereby initiating a cascade of intracellular signaling events. This phosphorylation facilitates bidirectional signaling between ephrin-B1 and EphB receptors, influencing various cellular processes such as cell attachment and migration. ephrin-B1 presence in the developing retina is particularly significant, as ephrin-B1 plays a vital role in retinal axon mapping along the dorsal-ventral axis, which is essential for proper visual system development. Understanding ephrin-B1 interactions and signaling pathways provides insights into developmental biology and potential roles in diseases where these pathways are disrupted.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ephrin-B1 Antibody (C-6) References:
- Eph receptors and ephrins restrict cell intermingling and communication. | Mellitzer, G., et al. 1999. Nature. 400: 77-81. PMID: 10403252
- Eph receptors and ephrins. | Jensen, PL. 2000. Stem Cells. 18: 63-4. PMID: 10661574
- In vivo tyrosine phosphorylation sites of activated ephrin-B1 and ephB2 from neural tissue. | Kalo, MS., et al. 2001. J Biol Chem. 276: 38940-8. PMID: 11466320
- Ephrin-B1 transduces signals to activate integrin-mediated migration, attachment and angiogenesis. | Huynh-Do, U., et al. 2002. J Cell Sci. 115: 3073-81. PMID: 12118063
- Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells. | Nagashima, K., et al. 2002. Mol Biol Cell. 13: 4231-42. PMID: 12475948
- Ephrin-B1 reverse signaling activates JNK through a novel mechanism that is independent of tyrosine phosphorylation. | Xu, Z., et al. 2003. J Biol Chem. 278: 24767-75. PMID: 12709432
- Tiam1 mediates neurite outgrowth induced by ephrin-B1 and EphA2. | Tanaka, M., et al. 2004. EMBO J. 23: 1075-88. PMID: 14988728
- Mutations of ephrin-B1 (EFNB1), a marker of tissue boundary formation, cause craniofrontonasal syndrome. | Twigg, SR., et al. 2004. Proc Natl Acad Sci U S A. 101: 8652-7. PMID: 15166289
- RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis. | Zhang, H., et al. 2021. Autophagy. 17: 3030-3047. PMID: 33280498
- Ephrin B1 Regulates Inflammatory Pathways in Retinal Müller Cells. | Liu, L., et al. 2024. J Diabetes Clin Res. 6: 1-7. PMID: 38501146
- ZIP4 inhibits Ephrin-B1 ubiquitination, activating Wnt5A/JNK/ZEB1 to promote liver cancer metastasis. | Guo, H., et al. 2024. Genes Dis. 11: 101312. PMID: 39040931
- Graded and lamina-specific distributions of ligands of EphB receptor tyrosine kinases in the developing retinotectal system. | Braisted, JE., et al. 1997. Dev Biol. 191: 14-28. PMID: 9356168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ephrin-B1 Antibody (C-6) | sc-515264 | 200 µg/ml | $316.00 | |||
ephrin-B1 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-528011 | 200 µg Ab; 10 µg BP | $354.00 | |||
ephrin-B1 Antibody (C-6): m-IgG1 BP-HRP Bundle | sc-533384 | 200 µg Ab; 20 µg BP | $354.00 | |||
ephrin-B1 Antibody (C-6) AC | sc-515264 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ephrin-B1 Antibody (C-6) HRP | sc-515264 HRP | 200 µg/ml | $316.00 | |||
ephrin-B1 Antibody (C-6) FITC | sc-515264 FITC | 200 µg/ml | $330.00 | |||
ephrin-B1 Antibody (C-6) PE | sc-515264 PE | 200 µg/ml | $343.00 | |||
ephrin-B1 Antibody (C-6) Alexa Fluor® 488 | sc-515264 AF488 | 200 µg/ml | $357.00 | |||
ephrin-B1 Antibody (C-6) Alexa Fluor® 546 | sc-515264 AF546 | 200 µg/ml | $357.00 | |||
ephrin-B1 Antibody (C-6) Alexa Fluor® 594 | sc-515264 AF594 | 200 µg/ml | $357.00 | |||
ephrin-B1 Antibody (C-6) Alexa Fluor® 647 | sc-515264 AF647 | 200 µg/ml | $357.00 | |||
ephrin-B1 Antibody (C-6) Alexa Fluor® 680 | sc-515264 AF680 | 200 µg/ml | $357.00 | |||
ephrin-B1 Antibody (C-6) Alexa Fluor® 790 | sc-515264 AF790 | 200 µg/ml | $357.00 |