Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EphB1 Double Nickase Plasmid (h): sc-401429-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EphB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EphB1 Double Nickase Plasmid (h) and EphB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPHB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EphB1 Antibody (5F10A4): sc-130054
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EphB1 Double Nickase Plasmid (h) | sc-401429-NIC | 20 µg | $410.00 | |||
EphB1 Double Nickase Plasmid (h2) | sc-401429-NIC-2 | 20 µg | $410.00 |
EPHB1 encodes EphB1, a receptor tyrosine kinase of the Eph family that binds membrane-tethered ephrin-B ligands to drive contact-dependent signaling. EphB1 regulates axon guidance, synaptic organization, cell migration, and boundary formation through bidirectional signaling and downstream modulation of Rho family GTPases, MAPK/ERK cascades, and PI3K-associated pathways. In epithelial and neuronal contexts, EphB1-dependent cues influence adhesion dynamics and cytoskeletal remodeling, shaping tissue architecture and cell positioning. Dysregulated EphB1 signaling has been linked to altered developmental patterning and has been investigated in cancer biology for roles in invasiveness, tumor microenvironment interactions, and pathway crosstalk.
EphB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPHB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPHB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPHB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPHB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.