EPCR Double Nickase Plasmid (h): sc-402158-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EPCR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EPCR Double Nickase Plasmid (h) and EPCR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PROCR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EPCR Antibody (RCR-49): sc-53982
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EPCR Double Nickase Plasmid (h) | sc-402158-NIC | 20 µg | $410.00 | |||
EPCR Double Nickase Plasmid (h2) | sc-402158-NIC-2 | 20 µg | $410.00 |
PROCR encodes endothelial protein C receptor (EPCR), a type I membrane glycoprotein predominantly expressed on vascular endothelium that binds protein C and activated protein C to localize anticoagulant and cytoprotective signaling at the cell surface. EPCR cooperates with thrombomodulin-thrombin–mediated protein C activation and supports protease-activated receptor–dependent pathways that influence endothelial barrier function, inflammation, and leukocyte trafficking. Altered EPCR expression or shedding can perturb hemostatic balance and vascular homeostasis, linking PROCR biology to thrombosis, inflammatory vasculopathies, and endothelial dysfunction. As a surface receptor with defined ligand interactions, EPCR is also used as a mechanistic readout for coagulation–inflammation crosstalk in cell and vessel models.
EPCR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PROCR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PROCR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PROCR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PROCR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.