EPC2 CRISPR/Cas9 KO Plasmid (h): sc-407068
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EPC2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EPC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EPC2 CRISPR/Cas9 KO Plasmid (h) | sc-407068 | 20 µg | $397.00 |
Overview
EPC2 (enhancer of polycomb homolog 2) encodes a chromatin-associated protein that functions as a component of Polycomb group regulatory networks, contributing to epigenetic control of transcription. EPC2 participates in multiprotein complexes that influence histone modification states and chromatin accessibility, thereby shaping programs governing cell identity, proliferation, and differentiation. Through these mechanisms, EPC2 links chromatin remodeling to transcriptional repression/activation dynamics across developmentally regulated loci. Altered EPC2-dependent epigenetic regulation has been investigated in the context of dysregulated gene expression signatures observed in cancer and other disorders with aberrant chromatin control.
EPC2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EPC2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EPC2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EPC2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EPC2 protein expression.
This CRISPR knockout system enables efficient generation of EPC2-deficient cell models for investigation of EPC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EPC2 exon(s) critical for EPC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EPC2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EPC2 CRISPR/Cas9 KO Plasmid (h) and EPC2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EPC2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EPC2 HDR Plasmid (h) and EPC2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EPC2 homology arms to support homology-directed repair at defined EPC2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.