Date published: 2025-9-5
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
EPAS-1/HIF-2 alpha Antibody (190b) - Western Blotting - Image 361754
EPAS-1 抗体 (190b): sc-13596. 对 Raji 全细胞裂解液中 EPAS-1 表达的 Western 印迹分析.
EPAS-1 抗体 (190b): sc-13596. 对 Raji 全细胞裂解液中 EPAS-1 表达的 Western 印迹分析.

EPAS-1/HIF-2 alpha 抗体 (190b): sc-13596

3.8(12)
寫評論提問

说明书
  • EPAS-1/HIF-2 alpha 抗体 190b 是小鼠单克隆 IgG1 κ，EPAS-1/HIF-2 alpha抗体, 在61篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组EPAS-1
  • EPAS-1/HIF-2 alpha 抗体 (190b) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 EPAS-1
  • 抗EPAS-1/HIF-2 alpha抗体（190b）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13596 X, 200 µg/0.1 ml)
  • 关于如何获取EPAS-1/HIF-2 alpha (190b): sc-13596的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 EPAS-1/HIF-2 alpha 抗体 (190b) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EPAS-1/HIF-2 alpha 抗体 (190b) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

EPAS-1抗体（190b）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的EPAS-1蛋白。EPAS-1 (190b) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。EPAS-1 蛋白（也称为 HIF-2α）在细胞对缺氧（一种以低氧水平为特征的状态）的反应中起着至关重要的作用。EPAS-1主要位于细胞核中，作为转录因子调节血管生成、红细胞生成和葡萄糖代谢等关键过程的基因表达。EPAS-1能够与其他缺氧诱导因子（如HIF-1α和HIF-3α）形成异二聚体，从而增强调节能力，使细胞能够有效适应氧含量的波动。这种适应性对于维持细胞内环境稳定以及支持细胞在各种生理和病理条件（包括癌症和缺血性疾病）下的存活至关重要。EPAS-1单克隆抗体（190b）使研究人员能够深入了解缺氧的机理及其对细胞功能和疾病进展的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EPAS-1/HIF-2 alpha 抗体 (190b) 参考文献:

  1. 转录因子EPAS-1/低氧诱导因子2α在血管重塑中起着重要作用。  |  Peng, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 8386-91. PMID: 10880563
  2. 缺氧：通过EPAS-1基因（编码HIF-2α）突变来适应高海拔环境。  |  van Patot, MC. and Gassmann, M. 2011. High Alt Med Biol. 12: 157-67. PMID: 21718164
  3. EPAS-1介导SP-1依赖的FBI-1表达，并调节肿瘤细胞的存活和增殖。  |  Wang, X., et al. 2014. Int J Mol Sci. 15: 15689-99. PMID: 25192290
  4. EPAS-1/HIF-2α和PXR信号通路之间的相互影响可调节胃癌细胞的多药耐药性。  |  Zhao, J., et al. 2016. Int J Biochem Cell Biol. 72: 73-88. PMID: 26783937
  5. EPAS 1、先天性心脏病和高海拔：遗传学、生物信息学和实验胚胎学的披露。  |  Sergi, C. 2019. Biosci Rep. 39: PMID: 31015364
  6. EPAS-1在肾透明细胞癌中的表达和预后意义。  |  Chen, B., et al. 2021. Ann Ital Chir. 92: 671-675. PMID: 34807005
  7. 缺氧诱导因子 1 是一种基本螺旋环-螺旋环-PAS 异二聚体，受细胞氧气张力调节。  |  Wang, GL., et al. 1995. Proc Natl Acad Sci U S A. 92: 5510-4. PMID: 7539918
  8. 内皮 PAS 结构域蛋白 1 (EPAS1)，一种选择性在内皮细胞中表达的转录因子。  |  Tian, H., et al. 1997. Genes Dev. 11: 72-82. PMID: 9000051
  9. 小鼠 Hif-1 alpha 基因座的分子特征。  |  Luo, G., et al. 1997. Gene Expr. 6: 287-99. PMID: 9368100
  10. 选择和分析缺氧诱导因子-1 alpha-亚基（HIF-1alpha）缺陷的突变细胞系。鉴定依赖和不依赖 HIF-1alpha 的缺氧诱导基因的表达。  |  Wood, SM., et al. 1998. J Biol Chem. 273: 8360-8. PMID: 9525945
  11. HIF-1alpha 在缺氧介导的细胞凋亡, 细胞增殖和肿瘤血管生成中的作用。  |  Carmeliet, P., et al. 1998. Nature. 394: 485-90. PMID: 9697772
  12. 第三个α-类缺氧诱导因子亚基 HIF3alpha 的分子特征和染色体定域。  |  Gu, YZ., et al. 1998. Gene Expr. 7: 205-13. PMID: 9840812

订购信息

产品名称产品编号规格价格数量收藏夹

EPAS-1/HIF-2 alpha 抗体 (190b)

sc-13596
200 µg/ml
$316.00

EPAS-1/HIF-2 alpha (190b): m-IgG Fc BP-HRP 套装

sc-528291
200 µg Ab; 10 µg BP
$354.00

EPAS-1/HIF-2 alpha (190b): m-IgGκ BP-HRP 套装

sc-520632
200 µg Ab, 40 µg BP
$354.00

EPAS-1/HIF-2 alpha (190b): m-IgG1 BP-HRP 套装

sc-542853
200 µg Ab; 20 µg BP
$354.00

EPAS-1/HIF-2 alpha 抗体 (190b) X

sc-13596 X
200 µg/0.1 ml
$316.00

EPAS-1/HIF-2 alpha 抗体 (190b) AC

sc-13596 AC
500 µg/ml, 25% agarose
$416.00

EPAS-1/HIF-2 alpha 抗体 (190b) HRP

sc-13596 HRP
200 µg/ml
$316.00

EPAS-1/HIF-2 alpha 抗体 (190b) FITC

sc-13596 FITC
200 µg/ml
$330.00

EPAS-1/HIF-2 alpha 抗体 (190b) PE

sc-13596 PE
200 µg/ml
$343.00

EPAS-1/HIF-2 alpha 抗体 (190b) Alexa Fluor® 488

sc-13596 AF488
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (190b) Alexa Fluor® 546

sc-13596 AF546
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (190b) Alexa Fluor® 594

sc-13596 AF594
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (190b) Alexa Fluor® 647

sc-13596 AF647
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (190b) Alexa Fluor® 680

sc-13596 AF680
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (190b) Alexa Fluor® 790

sc-13596 AF790
200 µg/ml
$357.00

请问该抗体室温放置24H后还可否继续使用

Asked by: luojuan
感谢您的提问。EPAS-1/HIF-2 alpha 抗体 (190b): sc-13596 正确的储存温度为4°C，千万不能冷冻。在室温下放置24h，对它的影响不大，可以继续使用。 Thank you for your question. EPAS-1/HIF-2 alpha antibody (190b): sc-13596 should be sotred at 4°C, do not freeze. It is OK to leave it at room temperature for 24 hours, it still can work normally.
Answered by: Technical Service SH
Date published: 2024-12-11

What is recommended incubation time for western blot of cell extracts with sc-13596? Thank You.

Asked by: KateDeck
Thank you for your question. We recommend incubating the primary antibody for 1 hour at room temperature (or overnight at 4C) in 5% nonfat milk in TBST.
Answered by: Technical Support
Date published: 2022-03-29

When should the TransCruz reagent sc-13596 X be used instead of the standard form sc-13596? What is the difference between the two forms?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration. The TransCruz reagent is provided as 10x more concentrated than the regular form: sc-13596 X, 200 µg/0.1 ml.
Answered by: Technical Support 9
Date published: 2017-01-12

Can this antibody be used for ChIP?

Asked by: TinTin
Thank you for your question! Yes, this antibody is recommeded for IP, which includes ChIP and CoIP. For more information, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Technical Support 9
Date published: 2017-01-12
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_13596, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 112ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from GoodI tested this antibody for western blotting on whole lysate of renal carcinoma cell line 786-0 (30 µg). I used 1:500 dilution in 2.5% milk dissolved in TBS-0.1%Tween incubated over-night at 4°C. The antibody detects a double band at the expected MW. Aspecific or post-traslational modification?
Date published: 2024-08-05
Rated 5 out of 5 by from Short terms of deliveryIt took only a week to come. The client is happy. Thank you.
Date published: 2022-03-01
Rated 1 out of 5 by from nessuna bandaho provato l'anticorpo in wb su campioni di H9c2 a C28I2, controllo e trattato con deferoxamina per simulare l'ipossia. Ho diluito l'anticorpo 1:200. Non ho rilevato alcuna banda nel caso delle C28I2, mentre nelle H9c2 compare una banda ma ad un peso molecolare molto diverso da quello atteso (circa 45-50 kDa).
Date published: 2021-06-24
Rated 4 out of 5 by from 良いです。内在性タンパク質を検出できますが、そのためには１次抗体をCanget signalで希釈する必要があります。
Date published: 2020-10-20
Rated 1 out of 5 by from 在小鼠肾脏组织中没有杂到目标条带。这抗体在肾脏组织中没有杂到目标条带，在70KD以上都没有任何条带，但在50~30KD之间有许多杂带，。
Date published: 2017-11-27
Rated 5 out of 5 by from GreatI am happy to get chance to try it. Will let you know how it is in rat sample.
Date published: 2017-07-05
Rated 4 out of 5 by from good band.Band had seen in hypoxic condition at 1:200 dilution in western blot.
Date published: 2017-06-16
Rated 4 out of 5 by from Buon funzionamentoSebbene scettica ho provato che questo anticorpo funziona
Date published: 2017-03-22
  • y_2025, m_8, d_27, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_13596, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
EPAS-1/HIF-2 alpha 抗体 (190b) is rated 3.8 out of 5 by 12.
  • y_2025, m_8, d_27, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_13596, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 101ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。