Epac2 Antibody (A-7): sc-28326

Epac2 Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Epac2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Epac2 antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Epac2, also known as Exchange Protein Directly Activated By cAMP 2, plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor that activates the Ras family GTPases Rap1 and Rap2 in a cAMP-dependent manner. This activation is vital for mediating various physiological responses, including gene expression, cell growth, differentiation, secretion, and neurotransmission, making Epac2 an important player in the regulation of numerous biological processes. Epac2 is predominantly expressed in the brain and adrenal gland, with significant levels found in regions such as the cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus, and cerebellum, highlighting potential involvement in neuroendocrine functions and cognitive processes. Human EPAC2 is encoded by a gene that contains at least 31 exons and is located on chromosome 2q31, with a prominent 4.4-kb transcript that underscores importance in both health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Epac2 Antibody (A-7) References:

1. Mechanism of regulation of the Epac family of cAMP-dependent RapGEFs.  |  de Rooij, J., et al. 2000. J Biol Chem. 275: 20829-36. PMID: 10777494
2. Characterization of the gene EPAC2: structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform.  |  Ueno, H., et al. 2001. Genomics. 78: 91-8. PMID: 11707077
3. Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of cAMP-GEFII.Rim2. Piccolo complex in cAMP-dependent exocytosis.  |  Fujimoto, K., et al. 2002. J Biol Chem. 277: 50497-502. PMID: 12401793
4. Structure and functional roles of Epac2 (Rapgef4).  |  Sugawara, K., et al. 2016. Gene. 575: 577-83. PMID: 26390815
5. Urinary concentrating defect in mice lacking Epac1 or Epac2.  |  Cherezova, A., et al. 2019. FASEB J. 33: 2156-2170. PMID: 30252533
6. Loss of EPAC2 alters dendritic spine morphology and inhibitory synapse density.  |  Jones, KA., et al. 2019. Mol Cell Neurosci. 98: 19-31. PMID: 31059774
7. Epac-2 ameliorates spontaneous colitis in Il-10-/- mice by protecting the intestinal barrier and suppressing NF-κB/MAPK signalling.  |  Song, X., et al. 2022. J Cell Mol Med. 26: 216-227. PMID: 34862717
8. β-Arrestin 2 and Epac2 Cooperatively Mediate DRD1-Stimulated Proliferation of Human Neural Stem Cells and Growth of Human Cerebral Organoids.  |  Dong, X., et al. 2022. Stem Cells. 40: 857-869. PMID: 35772103
9. Epac2 in midbrain dopamine neurons contributes to cocaine reinforcement via enhancement of dopamine release.  |  Liu, X., et al. 2022. Elife. 11: PMID: 35993549
10. Correction to Epac-2 ameliorates spontaneous colitis in Il-10-/- mice by protecting the intestinal barrier and suppressing NF-κB/MAPK signalling.  |  . 2023. J Cell Mol Med. 27: 3419-3421. PMID: 37608760
11. Novel crosstalk mechanisms between GluA3 and Epac2 in synaptic plasticity and memory in Alzheimer's disease.  |  Zhang, T., et al. 2024. Neurobiol Dis. 191: 106389. PMID: 38142840
12. A family of cAMP-binding proteins that directly activate Rap1.  |  Kawasaki, H., et al. 1998. Science. 282: 2275-9. PMID: 9856955

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Epac2 Antibody (A-7) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Epac2 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Epac2 Antibody (A-7) Specifications

Alternate Product Name: RAPGEF4

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-220

Epitope Target: Epac2

Epitope Species: human

Product Reactivity/Detection: Epac2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Epac2 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin