EP3 Antibody (5F5): sc-57105

EP3 Antibody (5F5) is a mouse monoclonal IgG2a kappa light chain antibody that detects EP3 protein of mouse, rat, human, and bovine origin by western blotting (WB) and immunoprecipitation (IP). EP3 (5F5) antibody is available as the non-conjugated anti-EP3 antibody. EP3 protein plays a crucial role in regulating renal function and blood pressure by mediating prostaglandin E2 effects, a key lipid mediator derived from arachidonic acid metabolism. EP3 protein is predominantly expressed in renal vessels, the thick ascending limb, and the collecting duct, where EP3 exists in at least six alternative splice variants that couple to Gi proteins. These variants inhibit cyclic adenosine monophosphate (cAMP) levels, leading to a decrease in sodium and water transport, which is vital for maintaining fluid balance and blood pressure homeostasis. EP3 protein is also involved in uterine smooth muscle contraction, highlighting EP3′s importance in reproductive physiology. Understanding EP3 function and regulation is essential for elucidating EP3′s role in various physiological and pathological processes, including hypertension and renal disorders.

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EP3 Antibody (5F5) References:

1. Prostaglandin E receptors and the kidney.  |  Breyer, MD. and Breyer, RM. 2000. Am J Physiol Renal Physiol. 279: F12-23. PMID: 10894784
2. Selective activation of E-type prostanoid(3)-receptors reduces myocardial infarct size. A novel insight into the cardioprotective effects of prostaglandins.  |  Thiemermann, C. and Zacharowski, K. 2000. Pharmacol Ther. 87: 61-7. PMID: 10924742
3. Expression of prostaglandin E receptor EP4 subtype in rat adrenal zona glomerulosa: involvement in aldosterone release.  |  Muro, S., et al. 2000. Endocr J. 47: 429-36. PMID: 11075723
4. Multiple signal transduction pathways through two prostaglandin E receptor EP3 subtype isoforms expressed in human uterus.  |  Kotani, M., et al. 2000. J Clin Endocrinol Metab. 85: 4315-22. PMID: 11095474
5. G protein-coupled prostanoid receptors and the kidney.  |  Breyer, MD. and Breyer, RM. 2001. Annu Rev Physiol. 63: 579-605. PMID: 11181968
6. EP3 activation facilitates bladder excitability via HCN channels on ICCs.  |  Wu, C., et al. 2017. Biochem Biophys Res Commun. 485: 535-541. PMID: 28131828
7. The role of EP3-receptor expression in cervical dysplasia.  |  Hester, A., et al. 2019. J Cancer Res Clin Oncol. 145: 313-319. PMID: 30402741
8. EP3 receptor is a prognostic factor in TA-MUC1-negative ovarian cancer.  |  Czogalla, B., et al. 2019. J Cancer Res Clin Oncol. 145: 2519-2527. PMID: 31485769
9. EP3 Is an Independent Prognostic Marker Only for Unifocal Breast Cancer Cases.  |  Zati Zehni, A., et al. 2020. Int J Mol Sci. 21: PMID: 32580276
10. EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.  |  Tao, X., et al. 2022. J Exp Med. 219: PMID: 35420633
11. [Molecular biology of prostaglandin E receptors--expression of multi-function by PGE receptor subtypes and isoforms].  |  Ichikawa, A. 1998. Nihon Rinsho. 56: 1813-8. PMID: 9702058
12. Regulation of renal function by prostaglandin E receptors.  |  Breyer, MD., et al. 1998. Kidney Int Suppl. 67: S88-94. PMID: 9736261

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EP3 Antibody (5F5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-EP3 Antibody (5F5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EP3 Antibody (5F5) Specifications

Alternate Product Name: EP3

Product Clone Name: 5F5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: EP3

Epitope Species: human

Product Reactivity/Detection: EP3

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG_2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for EP3 Antibody (5F5): N/A