ENSA Antibody (L7Q): sc-81883

ENSA Antibody (L7Q) is a mouse monoclonal IgG2a kappa light chain antibody that detects ENSA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ENSA antibody (L7Q) is available as the non-conjugated format. ENSA, also known as alpha-endosulfine or ARPP-19e, plays a crucial role in regulating ATP-dependent potassium (K(ATP)) channels, which are essential for maintaining cell membrane polarity and influencing cellular metabolism and insulin secretion. K(ATP) channels, composed of a Kir6.0 subfamily component and a sulfonylurea receptor (SUR) component, close in response to elevated ATP levels during increased glucose metabolism, thereby stimulating insulin secretion. ENSA serves as an endogenous ligand for the SUR component, inhibiting sulfonylurea binding and consequently reducing channel activity, which further promotes insulin release. ENSA is predominantly localized in the cytoplasm and exhibits high expression levels in brain and muscle tissues, while showing lower expression in the pancreas. Additionally, ENSA undergoes phosphorylation by protein kinase A (PKA) and exists in two isoforms, alpha and beta, generated through alternative splicing, highlighting complex regulatory mechanisms and functional diversity in cellular processes.

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ENSA Antibody (L7Q) References:

1. Isolation, characterization, and chromosomal localization of the human ENSA gene that encodes alpha-endosulfine, a regulator of beta-cell K(ATP) channels.  |  Héron, L., et al. 1999. Diabetes. 48: 1873-6. PMID: 10480622
2. Decreased alpha-endosulfine, an endogenous regulator of ATP-sensitive potassium channels, in brains from adult Down syndrome patients.  |  Kim, SH. and Lubec, G. 2001. J Neural Transm Suppl. 1-9. PMID: 11771735
3. Endosulfines: Novel regulators of insulin secretion.  |  Bataille, D. 2000. Drug News Perspect. 13: 453-62. PMID: 12937617
4. Alpha-endosulfine, a positional and functional candidate gene for type 2 diabetes: molecular screening, association studies, and role in reduced insulin secretion.  |  Wang, H., et al. 2004. Mol Genet Metab. 81: 9-15. PMID: 14728986
5. The transcribed endosulfine alpha gene is located within a type 2 diabetes-linked region on 1q: sequence and expression analysis in Pima Indians.  |  Thameem, F., et al. 2004. Mol Genet Metab. 81: 16-21. PMID: 14728987
6. Molecular characterization of a local sulfonylurea system in human adipose tissue.  |  Gabrielsson, BG., et al. 2004. Mol Cell Biochem. 258: 65-71. PMID: 15030171
7. ENSA expression correlates with attenuated tumor propagation in liver cancer.  |  Chen, YL., et al. 2013. Biochem Biophys Res Commun. 442: 56-61. PMID: 24211627
8. Ensa controls S-phase length by modulating Treslin levels.  |  Charrasse, S., et al. 2017. Nat Commun. 8: 206. PMID: 28785014
9. ENSA and ARPP19 differentially control cell cycle progression and development.  |  Hached, K., et al. 2019. J Cell Biol. 218: 541-558. PMID: 30626720
10. Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis.  |  Chen, YY., et al. 2022. Nat Commun. 13: 791. PMID: 35145111
11. The MASTL-ENSA-PP2A/B55 axis modulates cisplatin resistance in oral squamous cell carcinoma.  |  Gouttia, OG., et al. 2022. Front Cell Dev Biol. 10: 904719. PMID: 36247015
12. Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive KATP channel: molecular cloning, expression and biological properties.  |  Heron, L., et al. 1998. Proc Natl Acad Sci U S A. 95: 8387-91. PMID: 9653196