Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ENOPH1 Double Nickase Plasmid (m): sc-426793-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ENOPH1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ENOPH1 Double Nickase Plasmid (m) and ENOPH1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Enoph1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ENOPH1 Antibody (H-10): sc-365155
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENOPH1 Double Nickase Plasmid (m) | sc-426793-NIC | 20 µg | $410.00 | |||
ENOPH1 Double Nickase Plasmid (m2) | sc-426793-NIC-2 | 20 µg | $410.00 |
Enoph1 encodes the mouse ENOPH1 enzyme, a metabolic protein implicated in intracellular redox and thiol-dependent processes, linking cellular energy status to detoxification and stress-adaptive pathways. ENOPH1 activity is commonly discussed in the context of glutathione-associated metabolism and related intermediates, with downstream effects on oxidative stress handling, mitochondrial function, and overall metabolic homeostasis. Perturbation of these processes can influence proliferation and survival programs in metabolically active tissues and is relevant to research on metabolic dysfunction and stress-associated phenotypes. As a result, Enoph1 is a useful target for dissecting how redox buffering and small-molecule metabolism shape cellular physiology in mouse models.
ENOPH1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Enoph1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Enoph1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Enoph1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Enoph1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.