ENL Antibody (D-6): sc-393196

ENL Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ENL of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ENL (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ENL, also known as MLLT1, plays a crucial role in transcriptional regulation and is involved in the formation of MLL fusion proteins, which are significant in the context of leukemia. ENL interacts with several key partners, including the MLL gene, which influences myelomonocytic differentiation. Fusion of ENL with MLL, resulting from chromosomal translocations, leads to the production of MLL-ENL fusion protein, commonly associated with infant acute leukemias. This interaction alters normal gene expression patterns, contributing to the pathogenesis of various leukemias. ENL activation of transcription from synthetic reporter genes in both lymphoid and myeloid cells underscores ENL′s importance in hematopoietic malignancies, making anti-ENL antibody (D-6) an essential tool for researchers studying these complex diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ENL Antibody (D-6) References:

1. Physical interaction and functional antagonism between the RNA polymerase II elongation factor ELL and p53.  |  Shinobu, N., et al. 1999. J Biol Chem. 274: 17003-10. PMID: 10358050
2. DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis.  |  Strissel, PL., et al. 2000. Hum Mol Genet. 9: 1671-9. PMID: 10861294
3. Loss-of-function mutation of the AF9/MLLT3 gene in a girl with neuromotor development delay, cerebellar ataxia, and epilepsy.  |  Pramparo, T., et al. 2005. Hum Genet. 118: 76-81. PMID: 16001262
4. Local gene density predicts the spatial position of genetic loci in the interphase nucleus.  |  Murmann, AE., et al. 2005. Exp Cell Res. 311: 14-26. PMID: 16202404
5. Continuous MLL-ENL expression is necessary to establish a 'Hox Code' and maintain immortalization of hematopoietic progenitor cells.  |  Horton, SJ., et al. 2005. Cancer Res. 65: 9245-52. PMID: 16230385
6. The human ALL-1/MLL/HRX antigen is predominantly localized in the nucleus of resting and proliferating peripheral blood mononuclear cells.  |  Ennas, MG., et al. 1997. Cancer Res. 57: 2035-41. PMID: 9158002
7. Factors regulating the transcriptional elongation activity of RNA polymerase II.  |  Shilatifard, A. 1998. FASEB J. 12: 1437-46. PMID: 9806752

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ENL Antibody (D-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ENL Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ENL Antibody (D-6) Specifications

Alternate Product Name: ENL

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 131-188

Epitope Target: ENL

Epitope Species: human

Product Reactivity/Detection: ENL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ENL Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin