engrailed/invected Antibody (4D9): sc-53019

engrailed/invected Antibody (4D9) is a mouse monoclonal IgG1 kappa light chain antibody that detects engrailed and invected proteins of Drosophila and Xenopus origin by immunofluorescence (IF) and immunohistochemistry. anti-engrailed/invected antibody (4D9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. engrailed, a homeodomain-containing transcription factor, plays a crucial role in Drosophila development by regulating segmentation and forming posterior compartments. engrailed expression is vital for proper segmentation and early developmental processes, including organizing the preblastoderm and facilitating neurogenesis. engrailed′s function as a transcription factor underscores its importance in gene regulation during development, making engrailed a key player in the intricate processes that govern organismal development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

engrailed/invected Antibody (4D9) References:

1. Axial mesendoderm refines rostrocaudal pattern in the chick nervous system.  |  Rowan, AM., et al. 1999. Development. 126: 2921-34. PMID: 10357936
2. A new Drosophila APC homologue associated with adhesive zones of epithelial cells.  |  Yu, X., et al. 1999. Nat Cell Biol. 1: 144-51. PMID: 10559900
3. Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).  |  Stauber, M., et al. 2000. Proc Natl Acad Sci U S A. 97: 10844-9. PMID: 10995461
4. Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida).  |  Harzsch, S. 2003. Dev Biol. 258: 44-56. PMID: 12781681
5. Co-regulation of invected and engrailed by a complex array of regulatory sequences in Drosophila.  |  Cheng, Y., et al. 2014. Dev Biol. 395: 131-43. PMID: 25172431
6. Expression of engrailed proteins in arthropods, annelids, and chordates.  |  Patel, NH., et al. 1989. Cell. 58: 955-68. PMID: 2570637
7. Sculpting epithelia with planar polarized actomyosin networks: Principles from Drosophila.  |  Harris, TJC. 2018. Semin Cell Dev Biol. 81: 54-61. PMID: 28760393
8. Mimicry diversification in Papilio dardanus via a genomic inversion in the regulatory region of engrailed-invected.  |  Timmermans, MJTN., et al. 2020. Proc Biol Sci. 287: 20200443. PMID: 32345166
9. Pair-rule expression patterns of even-skipped are found in both short- and long-germ beetles.  |  Patel, NH., et al. 1994. Nature. 367: 429-34. PMID: 8107801
10. The Drosophila engrailed and invected genes: partners in regulation, expression and function.  |  Gustavson, E., et al. 1996. Genetics. 142: 893-906. PMID: 8849895
11. Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila.  |  White, P., et al. 1998. J Cell Biol. 140: 183-95. PMID: 9425166
12. Growth and patterning from the engrailed interface.  |  Hidalgo, A. 1998. Int J Dev Biol. 42: 317-24. PMID: 9654015

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-engrailed/invected Antibody (4D9) has 2 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

engrailed/invected Antibody (4D9) Specifications

Alternate Product Name: engrailed/invected

Product Clone Name: 4D9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: engrailed

Epitope Species: E. coli

Product Reactivity/Detection: engrailed and invected

Species Reactivity/Detection: d, x

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for engrailed/invected Antibody (4D9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin