Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h): sc-403871-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h) and Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EDN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Endothelin-2/EDN2/ET-2 Antibody (3B4-1C5): sc-293248
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h) | sc-403871-NIC | 20 µg | $410.00 | |||
Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h2) | sc-403871-NIC-2 | 20 µg | $410.00 |
EDN2 encodes endothelin-2 (ET-2), a secreted vasoactive peptide of the endothelin family that signals primarily through endothelin receptors ETA (EDNRA) and ETB (EDNRB) to regulate smooth muscle contraction, vascular tone, and paracrine communication. ET-2 engages G protein–coupled signaling that modulates intracellular calcium flux, PKC/MAPK cascades, and downstream transcriptional programs affecting proliferation, migration, and extracellular matrix remodeling. EDN2 expression is tightly regulated in reproductive tissues and during hypoxia and inflammation, linking it to processes such as ovulation-associated follicular rupture, angiogenic responses, and immune cell recruitment. Dysregulated endothelin signaling has been implicated in cardiovascular remodeling, fibrotic phenotypes, and tumor-associated microenvironment changes, making EDN2 a useful node for mechanistic studies of GPCR-driven pathways.
Endothelin-2/EDN2/ET-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EDN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EDN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EDN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EDN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.