Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Endophilin II Double Nickase Plasmid (h): sc-401620-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Endophilin II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Endophilin II Double Nickase Plasmid (h) and Endophilin II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SH3GL1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Endophilin II Antibody (A-11): sc-365704
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Endophilin II Double Nickase Plasmid (h) | sc-401620-NIC | 20 µg | $410.00 | |||
Endophilin II Double Nickase Plasmid (h2) | sc-401620-NIC-2 | 20 µg | $410.00 |
SH3GL1 encodes endophilin II, an SH3 domain–containing adaptor that couples membrane curvature sensing to endocytosis by engaging proline-rich partners and lipid-binding modules during clathrin-mediated vesicle formation. Endophilin II participates in synaptic vesicle recycling and receptor internalization, linking membrane trafficking to actin remodeling and signaling outputs from growth factor and immune receptors. Through its role in endocytic pathway dynamics and protein–protein interaction networks, SH3GL1 has been studied in contexts where vesicle trafficking influences cell proliferation, differentiation, and stress responses. Altered SH3GL1 regulation or fusion events have been associated with hematologic malignancy–relevant signaling and trafficking phenotypes, making it a useful target for mechanistic studies in cancer biology and immune cell function.
Endophilin II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SH3GL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SH3GL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SH3GL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SH3GL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.