![Endoglycosidase [EndoS] Antibody (B-8) - Western Blotting - Image 300503](https://media.scbt.com/product/endoglycosidase-endos-antibody-b-8-western-blotting_30_05_b_300503.jpg)
![Endoglycosidase [EndoS] Antibody (B-8) - Western Blotting - Image 300503](https://media.scbt.com/product/endoglycosidase-endos-antibody-b-8-western-blotting_30_05_t_300503.jpg "Endoglycosidase [EndoS] Antibody (B-8): sc-398039. Western blot analysis of Endoglycosidase expression in Streptococcus equi (virulent) (A), Streptococcus equi (avirulent) (B), Rhodococcus equi (C) and Escherichia coli (D) whole cell lysates. Note lack of reactivity with S. equi virulent strain in lane A, and with unrelated bacterial lysates in lanes C and D.")
Endoglycosidase [EndoS] Antibody (B-8): sc-398039. Western blot analysis of Endoglycosidase expression in Streptococcus equi (virulent) (A), Streptococcus equi (avirulent) (B), Rhodococcus equi (C) and Escherichia coli (D) whole cell lysates. Note lack of reactivity with S. equi virulent strain in lane A, and with unrelated bacterial lysates in lanes C and D.
Endoglycosidase [EndoS] Antibody (B-8): sc-398039
- Endoglycosidase [EndoS] Antibody (B-8) is a mouse monoclonal IgG1 κ Endoglycosidase [EndoS] antibody provided at 200 µg/ml
- raised against amino acids 1-243 mapping at the N-terminus of Endoglycosidase (EndoS) of Streptococcus equi subsp. equi 4047 origin
- Endoglycosidase [EndoS] Antibody (B-8) is recommended for detection of Endoglycosidase (EndoS) of Streptococcus equi subsp. equi 4047 origin by WB, IP, IF and ELISA
- Anti-Endoglycosidase [EndoS] Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Endoglycosidase [EndoS] Antibody (B-8) for WB applications. These reagents are now offered in bundles with Endoglycosidase [EndoS] Antibody (B-8) (see ordering information below).
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Endoglycosidase [EndoS] Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Endoglycosidase of Streptococcus equi subsp. equi 4047 origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Endoglycosidase antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EndoSe plays a crucial role in the degradation of glycoproteins by cleaving N-linked glycans, which is essential for proper cell signaling and immune response. Endoglycosidase′s ability to modulate glycoprotein structures can significantly impact Streptococcus equi virulence, making EndoSe a vital target for research into bacterial pathogenesis and potential therapeutic interventions. With 1018 amino acids, Endoglycosidase′s structure facilitates enzymatic activity, underscoring EndoSe′s importance in both basic and applied microbiological research.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Endoglycosidase [EndoS] Antibody (B-8) References:
- Getting to grips with strangles: an effective multi-component recombinant vaccine for the protection of horses from Streptococcus equi infection. | Guss, B., et al. 2009. PLoS Pathog. 5: e1000584. PMID: 19763180
- Molecular characterisation of 'strangles' outbreaks in the UK: the use of M-protein typing of Streptococcus equi ssp. equi. | Ivens, PA., et al. 2011. Equine Vet J. 43: 359-64. PMID: 21492215
- Association of Streptococcus equi with equine monocytes. | Mérant, C., et al. 2011. Vet Immunol Immunopathol. 143: 83-6. PMID: 21752476
- Streptococcus equi: a pathogen restricted to one host. | Waller, AS., et al. 2011. J Med Microbiol. 60: 1231-1240. PMID: 21757503
- Streptococcus equi subspecies equi infection (strangles) in horses. | Boyle, A. 2011. Compend Contin Educ Vet. 33: E1-7; quiz E8. PMID: 21870348
- Antiphagocytic function of an IgG glycosyl hydrolase from Streptococcus equi subsp. equi and its use as a vaccine component. | Flock, M., et al. 2012. Infect Immun. 80: 2914-9. PMID: 22615244
- Development of a novel mucosal vaccine against strangles by supercritical enhanced atomization spray-drying of Streptococcus equi extracts and evaluation in a mouse model. | Rodrigues, MA., et al. 2012. Eur J Pharm Biopharm. 82: 392-400. PMID: 22841882
- Detection of Streptococcus equi subspecies equi using a triplex qPCR assay. | Webb, K., et al. 2013. Vet J. 195: 300-4. PMID: 22884566
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Endoglycosidase [EndoS] Antibody (B-8) | sc-398039 | 200 µg/ml | $322.00 | |||
Endoglycosidase [EndoS] Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-527731 | 200 µg Ab; 10 µg BP | $361.00 | |||
Endoglycosidase [EndoS] Antibody (B-8): m-IgG1 BP-HRP Bundle | sc-533104 | 200 µg Ab; 20 µg BP | $361.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) AC | sc-398039 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) HRP | sc-398039 HRP | 200 µg/ml | $322.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) FITC | sc-398039 FITC | 200 µg/ml | $336.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) PE | sc-398039 PE | 200 µg/ml | $349.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) Alexa Fluor® 488 | sc-398039 AF488 | 200 µg/ml | $364.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) Alexa Fluor® 546 | sc-398039 AF546 | 200 µg/ml | $364.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) Alexa Fluor® 594 | sc-398039 AF594 | 200 µg/ml | $364.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) Alexa Fluor® 647 | sc-398039 AF647 | 200 µg/ml | $364.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) Alexa Fluor® 680 | sc-398039 AF680 | 200 µg/ml | $364.00 | |||
Endoglycosidase [EndoS] Antibody (B-8) Alexa Fluor® 790 | sc-398039 AF790 | 200 µg/ml | $364.00 |