Endoglin/CD105 Double Nickase Plasmid (h): sc-400599-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Endoglin/CD105 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Endoglin/CD105 Double Nickase Plasmid (h) and Endoglin/CD105 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ENG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Endoglin/CD105 Antibody (P3D1): sc-18838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Endoglin/CD105 Double Nickase Plasmid (h) | sc-400599-NIC | 20 µg | $410.00 | |||
Endoglin/CD105 Double Nickase Plasmid (h2) | sc-400599-NIC-2 | 20 µg | $410.00 |
ENG encodes endoglin (CD105), a transmembrane co-receptor for TGF-β superfamily ligands that modulates ALK1/ALK5 signaling balance and downstream SMAD-dependent transcription. Endoglin is highly expressed on proliferating endothelial cells and participates in regulation of angiogenesis, vascular remodeling, extracellular matrix dynamics, and endothelial-to-mesenchymal transition. Through interactions with TGFBR2, ACVRL1, and associated signaling complexes, CD105 influences cellular adhesion and migration programs that shape vessel integrity. Dysregulated ENG function is linked to vascular pathobiology, including hereditary hemorrhagic telangiectasia and tumor-associated angiogenic microenvironments, making it a key target for mechanistic studies in endothelial biology.
Endoglin/CD105 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ENG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ENG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ENG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ENG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.