Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ENDOG Double Nickase Plasmid (h): sc-403263-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ENDOG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ENDOG Double Nickase Plasmid (h) and ENDOG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ENDOG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ENDOG Antibody (B-2): sc-365359
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENDOG Double Nickase Plasmid (h) | sc-403263-NIC | 20 µg | $410.00 | |||
ENDOG Double Nickase Plasmid (h2) | sc-403263-NIC-2 | 20 µg | $410.00 |
ENDOG encodes endonuclease G, a mitochondria-localized nuclease that participates in mitochondrial DNA metabolism and can contribute to nuclear DNA fragmentation under cellular stress. The protein has been linked to intrinsic apoptosis-related processes and mitochondrial homeostasis, interfacing with pathways governing oxidative stress responses and genome integrity. Altered ENDOG activity has been associated with phenotypes involving mitochondrial dysfunction and DNA damage sensitivity, making it relevant to studies of metabolic stress and cell death regulation. As a conserved nuclease, ENDOG is also used as a model factor for dissecting mitochondrial–nuclear signaling during stress-induced chromatin degradation.
ENDOG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ENDOG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ENDOG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ENDOG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ENDOG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.