Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ENDOG CRISPR/Cas9 KO Plasmid (m): sc-420176
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ENDOG CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ENDOG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ENDOG Antibody (B-2): sc-365359
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENDOG CRISPR/Cas9 KO Plasmid (m) | sc-420176 | 20 µg | $397.00 |
Overview
Endog encodes endonuclease G (ENDOG), a mitochondrial nuclease implicated in mitochondrial DNA metabolism and the controlled degradation of nucleic acids during cellular stress. ENDOG participates in pathways linked to apoptosis-like DNA fragmentation, mitochondrial genome maintenance, and broader mitochondrial homeostasis that can influence oxidative phosphorylation and reactive oxygen species signaling. Altered ENDOG activity has been associated with dysregulated mitochondrial function and genome integrity, processes relevant to models of neurodegeneration, cardiometabolic dysfunction, and inflammatory stress. In mouse systems, Endog provides a tractable node for interrogating mitochondria-to-nucleus communication and stress-responsive cell fate decisions.
ENDOG CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Endog gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Endog together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Endog open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ENDOG protein expression.
This CRISPR knockout system enables efficient generation of Endog-deficient cell models for investigation of ENDOG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Endog exon(s) critical for ENDOG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Endog genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ENDOG CRISPR/Cas9 KO Plasmid (m) and ENDOG CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Endog locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ENDOG HDR Plasmid (m) and ENDOG HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Endog homology arms to support homology-directed repair at defined Endog target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.