Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
endobrevin CRISPR/Cas9 KO Plasmid (h): sc-403438
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- endobrevin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the endobrevin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: endobrevin Antibody (G-12): sc-166820
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
endobrevin CRISPR/Cas9 KO Plasmid (h) | sc-403438 | 20 µg | $397.00 |
Overview
VAMP8 encodes endobrevin, a vesicle-associated SNARE that mediates membrane fusion events central to regulated exocytosis and endocytic trafficking. It participates in SNARE complex assembly with partner syntaxins and SNAP proteins to drive vesicle docking and fusion, supporting processes such as granule secretion, phagosome–lysosome fusion, and trafficking to late endosomes/lysosomes. In immune and epithelial contexts, VAMP8-dependent vesicle dynamics influence inflammasome-related secretion and mucosal barrier functions, linking its dysregulation to inflammatory phenotypes and altered host–pathogen responses. Perturbation of endobrevin can also remodel receptor recycling and degradative pathways, making it relevant to studies of signaling attenuation, antigen processing, and cellular stress responses.
endobrevin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VAMP8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VAMP8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VAMP8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish endobrevin protein expression.
This CRISPR knockout system enables efficient generation of VAMP8-deficient cell models for investigation of endobrevin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VAMP8 exon(s) critical for endobrevin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VAMP8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by endobrevin CRISPR/Cas9 KO Plasmid (h) and endobrevin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VAMP8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by endobrevin HDR Plasmid (h) and endobrevin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VAMP8 homology arms to support homology-directed repair at defined VAMP8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.