Emx2 CRISPR/Cas9 KO Plasmid (m): sc-420170
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Emx2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Emx2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Emx2 CRISPR/Cas9 KO Plasmid (m) | sc-420170 | 20 µg | $397.00 |
Overview
EMX2 (empty spiracles homeobox 2) is a homeodomain transcription factor that controls regional patterning and cell fate decisions during embryogenesis, with prominent roles in forebrain and cortical arealization as well as urogenital and limb development in mouse. It regulates developmental gene expression programs by binding cis-regulatory elements and coordinating networks that interface with WNT/β-catenin, FGF, and BMP morphogen signaling, influencing progenitor proliferation, differentiation, and tissue boundary formation. Altered Emx2 dosage or regulatory activity has been associated with neurodevelopmental abnormalities and structural malformations, and dysregulated EMX2 expression has been reported in multiple tumor contexts as a marker of aberrant differentiation states. These features make Emx2 a useful node for studying transcriptional control of developmental pathways and lineage specification.
Emx2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Emx2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Emx2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Emx2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Emx2 protein expression.
This CRISPR knockout system enables efficient generation of Emx2-deficient cell models for investigation of Emx2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Emx2 exon(s) critical for Emx2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Emx2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Emx2 CRISPR/Cas9 KO Plasmid (m) and Emx2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Emx2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Emx2 HDR Plasmid (m) and Emx2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Emx2 homology arms to support homology-directed repair at defined Emx2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.