Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EMSY Double Nickase Plasmid (h): sc-405722-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMSY Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EMSY Double Nickase Plasmid (h) and EMSY Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EMSY. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EMSY Antibody (E-9): sc-515469
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMSY Double Nickase Plasmid (h) | sc-405722-NIC | 20 µg | $410.00 | |||
EMSY Double Nickase Plasmid (h2) | sc-405722-NIC-2 | 20 µg | $410.00 |
EMSY encodes a nuclear chromatin-associated protein implicated in the regulation of DNA double-strand break repair through interactions with BRCA2 and modulation of homologous recombination. By influencing chromatin state and the DNA damage response, EMSY contributes to genome stability, replication stress signaling, and cell-cycle checkpoint control. Altered EMSY dosage or dysregulated expression has been linked to aberrant repair pathway choice and transcriptional programs relevant to oncogenic transformation and tumor progression. As a result, EMSY is frequently studied in the context of DNA repair networks, chromatin remodeling, and determinants of sensitivity to genotoxic stress in human cells.
EMSY Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EMSY locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EMSY. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EMSY function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EMSY-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.