EMP-3 CRISPR/Cas9 KO Plasmid (h): sc-407036
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMP-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EMP-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EMP-3 Antibody (SW-5): sc-81797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMP-3 CRISPR/Cas9 KO Plasmid (h) | sc-407036 | 20 µg | $397.00 |
Overview
EMP3 encodes epithelial membrane protein 3 (EMP-3), a tetraspan-like membrane protein implicated in regulation of cell adhesion, membrane organization, and signal transduction at the cell surface. In human cells, EMP-3 has been linked to modulation of pathways involved in proliferation and stress responses, including receptor-associated signaling and downstream MAPK/PI3K network components depending on cellular context. Altered EMP3 expression patterns have been reported across multiple tumor types and in neurological disease-related studies, supporting its use as a molecular variable in models of oncogenic signaling, invasion, and differentiation. As a membrane-associated factor, EMP-3 is also relevant for dissecting changes in cell–cell interactions and microenvironmental responses.
EMP-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EMP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EMP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EMP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EMP-3 protein expression.
This CRISPR knockout system enables efficient generation of EMP3-deficient cell models for investigation of EMP-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EMP3 exon(s) critical for EMP-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EMP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EMP-3 CRISPR/Cas9 KO Plasmid (h) and EMP-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EMP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EMP-3 HDR Plasmid (h) and EMP-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EMP3 homology arms to support homology-directed repair at defined EMP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.