Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EMMPRIN/CD147 Double Nickase Plasmid (m): sc-419369-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMMPRIN/CD147 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EMMPRIN/CD147 Double Nickase Plasmid (m) and EMMPRIN/CD147 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Bsg. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EMMPRIN/CD147 Antibody (B-5): sc-46700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMMPRIN/CD147 Double Nickase Plasmid (m) | sc-419369-NIC | 20 µg | $410.00 | |||
EMMPRIN/CD147 Double Nickase Plasmid (m2) | sc-419369-NIC-2 | 20 µg | $410.00 |
Basigin (Bsg), also known as EMMPRIN/CD147, is a broadly expressed immunoglobulin superfamily glycoprotein that regulates cell–cell interactions and membrane protein organization at the cell surface. In mouse cells it functions as an essential chaperone for monocarboxylate transporters (MCT1/MCT4), supporting lactate transport and glycolytic metabolism, and it modulates extracellular matrix remodeling through induction of matrix metalloproteinases in stromal and immune contexts. CD147 also participates in integrin- and MAPK-linked signaling that influences adhesion, migration, and inflammatory responses. Dysregulated Bsg/CD147 activity has been associated with tumor microenvironment remodeling, fibrotic and inflammatory phenotypes, and altered metabolic coupling in disease-relevant tissues.
EMMPRIN/CD147 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Bsg locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Bsg. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Bsg function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Bsg-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.