EMID1 Double Nickase Plasmid (m): sc-431233-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMID1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EMID1 Double Nickase Plasmid (m) and EMID1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Emid1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EMID1 Antibody (G-12): sc-390288
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMID1 Double Nickase Plasmid (m) | sc-431233-NIC | 20 µg | $410.00 | |||
EMID1 Double Nickase Plasmid (m2) | sc-431233-NIC-2 | 20 µg | $410.00 |
EMID1 (EMI domain containing 1) is a secreted extracellular matrix–associated protein implicated in organizing pericellular matrices and modulating cell–matrix interactions during development and tissue homeostasis in mouse. Through its ECM localization and predicted protein–protein interaction modules, EMID1 is studied in the context of adhesion-dependent signaling, basement membrane assembly, and regulation of morphogenetic processes that influence cell migration and differentiation. Dysregulation of ECM composition and remodeling is a common feature of fibrosis, developmental abnormalities, and tumor microenvironment changes, making Emid1 a relevant target for mechanistic studies of matrix-driven phenotypes. Mouse Emid1 models can help dissect how ECM components coordinate signaling outputs that affect tissue architecture and stress responses.
EMID1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Emid1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Emid1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Emid1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Emid1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.