Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Elongin A Antibody (A-5): sc-373811. Near-Infrared western blot analysis of Elongin A expression in HeLa (A) and Jurkat (B) nuclear extracts and Jurkat whole cell lysate (C). Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Elongin A CRISPR/Cas9 KO Plasmid (h): sc-404668
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Elongin A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Elongin A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Elongin A Antibody (A-5): sc-373811
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elongin A CRISPR/Cas9 KO Plasmid (h) | sc-404668 | 20 µg | $397.00 |