Elmo3 Antibody (E-4): sc-166364
- Elmo3 Antibody (E-4) is a mouse monoclonal IgG1 κ Elmo3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 64-95 within an internal region of Elmo3 of mouse origin
- Elmo3 Antibody (E-4) is recommended for detection of Elmo3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
- Anti-Elmo3 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Elmo3 Antibody (E-4) for WB applications. These reagents are now offered in bundles with Elmo3 Antibody (E-4) (see ordering information below).
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Elmo3 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Elmo3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Elmo3 antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Elmo3 plays a crucial role in regulating cytoskeletal dynamics, particularly during processes such as phagocytosis and cell motility. Elmo3 interacts with DOCK180 and Crk II, forming a complex essential for activating Rac and Rho small GTPases, which are pivotal in mediating cell shape changes and motility. Proper functioning of Elmo3 is vital for various cellular processes, including engulfment of apoptotic cells and formation of cellular protrusions, which are important for tissue homeostasis and immune responses. Elmo3′s involvement in these pathways highlights significance in developmental processes and potential implications in cancer metastasis, making Elmo3 a valuable target for research in cell biology and therapeutic development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Elmo3 Antibody (E-4) References:
- CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. | Gumienny, TL., et al. 2001. Cell. 107: 27-41. PMID: 11595183
- Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. | Brugnera, E., et al. 2002. Nat Cell Biol. 4: 574-82. PMID: 12134158
- DOCK2 regulates Rac activation and cytoskeletal reorganization through interaction with ELMO1. | Sanui, T., et al. 2003. Blood. 102: 2948-50. PMID: 12829596
- RhoG activates Rac1 by direct interaction with the Dock180-binding protein Elmo. | Katoh, H. and Negishi, M. 2003. Nature. 424: 461-4. PMID: 12879077
- PH domain of ELMO functions in trans to regulate Rac activation via Dock180. | Lu, M., et al. 2004. Nat Struct Mol Biol. 11: 756-62. PMID: 15247908
- Phagocytosis of apoptotic cells is regulated by a UNC-73/TRIO-MIG-2/RhoG signaling module and armadillo repeats of CED-12/ELMO. | deBakker, CD., et al. 2004. Curr Biol. 14: 2208-16. PMID: 15620647
- C-terminal SH3 domain of CrkII regulates the assembly and function of the DOCK180/ELMO Rac-GEF. | Akakura, S., et al. 2005. J Cell Physiol. 204: 344-51. PMID: 15700267
- Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck. | Yokoyama, N., et al. 2005. Biochemistry. 44: 8841-9. PMID: 15952790
- The DOCK180/Elmo complex couples ARNO-mediated Arf6 activation to the downstream activation of Rac1. | Santy, LC., et al. 2005. Curr Biol. 15: 1749-54. PMID: 16213822
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elmo3 Antibody (E-4) | sc-166364 | 200 µg/ml | $322.00 | |||
Elmo3 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-528949 | 200 µg Ab; 10 µg BP | $361.00 | |||
Elmo3 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-521577 | 200 µg Ab, 40 µg BP | $361.00 | |||
Elmo3 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-543229 | 200 µg Ab; 20 µg BP | $361.00 | |||
Elmo3 Antibody (E-4) AC | sc-166364 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Elmo3 Antibody (E-4) HRP | sc-166364 HRP | 200 µg/ml | $322.00 | |||
Elmo3 Antibody (E-4) FITC | sc-166364 FITC | 200 µg/ml | $336.00 | |||
Elmo3 Antibody (E-4) PE | sc-166364 PE | 200 µg/ml | $349.00 | |||
Elmo3 Antibody (E-4) Alexa Fluor® 488 | sc-166364 AF488 | 200 µg/ml | $364.00 | |||
Elmo3 Antibody (E-4) Alexa Fluor® 546 | sc-166364 AF546 | 200 µg/ml | $364.00 | |||
Elmo3 Antibody (E-4) Alexa Fluor® 594 | sc-166364 AF594 | 200 µg/ml | $364.00 | |||
Elmo3 Antibody (E-4) Alexa Fluor® 647 | sc-166364 AF647 | 200 µg/ml | $364.00 | |||
Elmo3 Antibody (E-4) Alexa Fluor® 680 | sc-166364 AF680 | 200 µg/ml | $364.00 | |||
Elmo3 Antibody (E-4) Alexa Fluor® 790 | sc-166364 AF790 | 200 µg/ml | $364.00 | |||
Elmo3 (E-4) Neutralizing Peptide | sc-166364 P | 100 µg/0.5 ml | $69.00 |