ELL3 CRISPR/Cas9 KO Plasmid (h): sc-405835
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ELL3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ELL3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ELL3 CRISPR/Cas9 KO Plasmid (h) | sc-405835 | 20 µg | $397.00 |
Overview
ELL3 (eleven-nineteen lysine-rich leukemia gene 3) encodes a transcription elongation factor–like protein related to the ELL family, which interfaces with RNA polymerase II to influence productive elongation and transcript output. Although less well characterized than other ELL paralogs, ELL3 is implicated in regulating gene-expression programs linked to cell identity, differentiation, and developmental timing through modulation of transcriptional dynamics. Altered control of elongation and RNA processing is a recurring feature of oncogenic and developmental dysregulation, making ELL3 a relevant target for studying how transcriptional machinery contributes to aberrant gene expression. In human cells, investigating ELL3 function can illuminate context-specific transcriptional control and its potential connections to disease-associated expression signatures.
ELL3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ELL3 protein expression.
This CRISPR knockout system enables efficient generation of ELL3-deficient cell models for investigation of ELL3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELL3 exon(s) critical for ELL3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ELL3 CRISPR/Cas9 KO Plasmid (h) and ELL3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ELL3 HDR Plasmid (h) and ELL3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELL3 homology arms to support homology-directed repair at defined ELL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.