Elf-4 Antibody (E-11): sc-515363
- Elf-4 Antibody (E-11) is a mouse monoclonal IgM κ Elf-4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 583-599 within an internal region of Elf-4 of human origin
- recommended for detection of Elf-4 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-515363 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Elf-4 Antibody (E-11) for WB applications. This reagent is now offered in a bundle with Elf-4 Antibody (E-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Elf-4 Antibody (E-11) is a mouse monoclonal IgM antibody that detects Elf-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Elf-4 antibody (E-11) is available as a non-conjugated format. Elf-4, also known as myeloid Elf-1-like factor, ELF4, or MEF, is a 663 amino acid member of the Ets-1 family of transcription factors, which are characterized by a conserved DNA binding domain that plays a crucial role in regulating cellular processes such as proliferation, differentiation, hematopoiesis, and angiogenesis. This Ets domain specifically binds to DNA sequences containing the consensus sequence 5′-WGGA-3′, known as the Ets-binding domain, allowing Elf-4 to activate the promoters of various hematopoietic growth factor genes, including GM-CSF, IL-3, and IL-8. Notably, Elf-4 is primarily localized in the nucleus and is highly expressed in placenta and myeloid leukemia cells, with lower expression levels observed in tissues such as the lung, heart, thymus, spleen, colon, ovary, and peripheral blood lymphocytes. Elf-4′s ability to activate the Perforin 1 promoter in natural killer (NK) cells suggests a potential involvement in tumorigenesis, highlighting Elf-4′s importance in both normal physiological processes and disease states.
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Elf-4 Antibody (E-11) References:
- Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias. | Mao, S., et al. 1999. Mol Cell Biol. 19: 3635-44. PMID: 10207087
- Myeloid ELF-1-like factor up-regulates lysozyme transcription in epithelial cells. | Kai, H., et al. 1999. J Biol Chem. 274: 20098-102. PMID: 10400620
- Functional dissection of the ETS transcription factor MEF. | Suico, MA., et al. 2002. Biochim Biophys Acta. 1577: 113-20. PMID: 12151102
- The ETS protein MEF plays a critical role in perforin gene expression and the development of natural killer and NK-T cells. | Lacorazza, HD., et al. 2002. Immunity. 17: 437-49. PMID: 12387738
- Myeloid ELF1-like factor is a potent activator of interleukin-8 expression in hematopoietic cells. | Hedvat, CV., et al. 2004. J Biol Chem. 279: 6395-400. PMID: 14625302
- Myeloid Elf-1-like factor, an ETS transcription factor, up-regulates lysozyme transcription in epithelial cells through interaction with promyelocytic leukemia protein. | Suico, MA., et al. 2004. J Biol Chem. 279: 19091-8. PMID: 14976184
- Tumor promoting properties of the ETS protein MEF in ovarian cancer. | Yao, JJ., et al. 2007. Oncogene. 26: 4032-7. PMID: 17213815
- MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties. | Miyazaki, Y., et al. 1996. Oncogene. 13: 1721-9. PMID: 8895518
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elf-4 Antibody (E-11) | sc-515363 | 200 µg/ml | $316.00 | |||
Elf-4 Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-552185 | 200 µg Ab; 40 µg BP | $354.00 | |||
Elf-4 (E-11) Neutralizing Peptide | sc-515363 P | 100 µg/0.5 ml | $68.00 | |||
Elf-4 Antibody (E-11) X | sc-515363 X | 200 µg/0.1 ml | $316.00 |