Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
elastin CRISPR/Cas9 KO Plasmid (h2): sc-400640-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- elastin CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the elastin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: elastin Antibody (E-11): sc-166543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
elastin CRISPR/Cas9 KO Plasmid (h2) | sc-400640-KO-2 | 20 µg | $397.00 |
Overview
Human ELN encodes elastin, a highly crosslinked extracellular matrix protein that provides elasticity and recoil to elastic fibers in arteries, lung, and skin. Elastin assembly involves microfibrillar scaffolds and lysyl oxidase–mediated crosslinking, supporting vascular compliance and tissue mechanics and influencing cell behavior through matrix–integrin signaling and mechanotransduction pathways. Disruption of ELN perturbs elastic fiber formation and extracellular matrix homeostasis, altering smooth muscle cell phenotype and connective tissue organization. ELN dysfunction is associated with disorders of vascular and connective tissue integrity, including supravalvular aortic stenosis, cutis laxa, and other elastin-related arteriopathies.
elastin CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the ELN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish elastin protein expression.
This CRISPR knockout system enables efficient generation of ELN-deficient cell models for investigation of elastin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELN exon(s) critical for elastin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by elastin CRISPR/Cas9 KO Plasmid (h) and elastin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by elastin HDR Plasmid (h) and elastin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELN homology arms to support homology-directed repair at defined ELN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.